Arteriosclerosis and cancer detection method using deoxyhypusine synthase gene as indicator

Abstract

A method for detecting anti-deoxyhypusine synthase autoantibody in a subject, wherein the method includes detecting, in a blood, serum, or plasma sample from a test subject with digestive system cancer, the amount of anti-deoxyhypusine synthase autoantibody, wherein the autoantibody is able to bind to a polypeptide consisting of the amino acid sequence of SEQ ID NO: 1.

1 . A method for detecting anti-deoxyhypusine synthase autoantibody in a subject, wherein said method comprises:
 detecting, in a blood, serum, or plasma sample from a test subject with digestive system cancer, the amount of anti-deoxyhypusine synthase autoantibody, wherein said autoantibody is able to bind to a polypeptide consisting of the amino acid sequence of SEQ ID NO: 1.   
     
     
         2 . The method according to  claim 1 , wherein said detecting comprises contacting said test sample with an immobilized polypeptide comprising the amino acid sequence of SEQ ID NO: 1, or comprising the amino acid sequence of residues 184-369 of SEQ ID NO: 1; and when said test sample contains antibody that binds to said immobilized polypeptide, binding between the antibody and the immobilized polypeptide is detected as a signal. 
     
     
         3 . The method according to  claim 1 , wherein the amount of the anti-deoxyhypusine synthase autoantibody is determined by performing enzyme-linked immunosorbent assay (ELISA), enzyme immunoassay (EIA), radioimmunoassay (RIA), western blotting, turbidimetry, nephelometry, latex agglutination turbidimetry, or chemiluminescence enzyme immunoassay (CLEIA). 
     
     
         4 . The method according to  claim 3 , wherein the amount of the anti-deoxyhypusine synthase autoantibody is determined by performing enzyme immunoassay (EIA). 
     
     
         5 . The method according to  claim 3 , wherein the amount of the anti-deoxyhypusine synthase autoantibody is determined by performing enzyme-linked immunosorbent assay (ELISA). 
     
     
         6 . The method according to  claim 1 , wherein the digestive system cancer is esophageal cancer or colorectal cancer.