Cell separation device and method for using same

Abstract

A cell separation device configured for separating cells from microcarriers or spheroids in a liquid is provided. The cell separation device includes a vessel comprising a first port, a second port, and a cavity; and a porous mesh disposed within the cavity to divide the cavity into a first compartment and a second compartment, wherein the first port is in communication with the first compartment of the cavity, the first port located to a first side of the porous mesh, wherein the second port is in communication with the second compartment of the cavity, the second port located to a second side of the porous mesh, and wherein the porous mesh is positioned within the cavity to have a substantially vertical orientation or an inclined orientation with respect to a flow of liquid through the porous mesh.

1 . A cell separation device configured for separating cells from microcarriers or spheroids in a liquid, the cell separation device comprising:
 a vessel comprising a first port, a second port, and a cavity; and   a porous mesh disposed within the cavity to divide the cavity into a first compartment and a second compartment,   wherein the first port is in communication with the first compartment of the cavity, the first port located to a first side of the porous mesh,   wherein the second port is in communication with the second compartment of the cavity, the second port located to a second side of the porous mesh, and   wherein the porous mesh is positioned within the cavity to have a substantially vertical orientation or an inclined orientation with respect to a flow of liquid through the porous mesh.   
     
     
         2 . The cell separation device of  claim 1 , wherein the first port is located at one end of the vessel and the second port is located at an opposing end of the vessel. 
     
     
         3 . The cell separation device of  claim 2 , wherein the first port and the second port are off-set from one another on the vessel such that the porous mesh has the substantially vertical orientation within the cavity when (1) the first port is located in a substantially upward orientation and the second port is located in a substantially downward orientation, or (2) the first port is located in the substantially downward orientation and the second port is located in the substantially upward orientation. 
     
     
         4 . The cell separation device of  claim 2 , wherein the first port and the second port are in-line with one another on the vessel such that the porous mesh has the inclined orientation within the cavity when (1) the first port is located in a substantially upward orientation and the second port is located in a substantially downward orientation, or (2) the first port is located in the substantially downward orientation and the second port is located in the substantially upward orientation. 
     
     
         5 . The cell separation device of  claim 1 , wherein the first port and the second port are both positioned on one side of the vessel and the porous mesh has the substantially vertical orientation within the cavity when (1) the first port and the second port are located in a substantially upward orientation, or (2) the first port and the second port are located in a substantially downward orientation. 
     
     
         6 . The cell separation device of  claim 1 , further comprising:
 a first cap attachable to the first port; and   a second cap attachable to the second port.   
     
     
         7 . The cell separation device of  claim 1 , further comprising:
 a first flow control system attached to the first port and external equipment; and   a second flow control system attached to the second port and external equipment.   
     
     
         8 . The cell separation device of  claim 1 , wherein the porous mesh comprises pores therein with sizes ranging from about 12 microns to about 200 microns. 
     
     
         9 . The cell separation device of  claim 1 , further comprising:
 an outer housing surrounding at least a portion of the vessel.   
     
     
         10 . The cell separation device of  claim 1 , further comprising:
 a ring stand coupled to the vessel, the ring stand configured to facilitate movement and rotation of the vessel.   
     
     
         11 - 20 . (canceled) 
     
     
         21 . A cell separation device, comprising:
 a vessel comprising a first port, a second port, and a cavity; and   a porous mesh disposed within the cavity to divide the cavity into a first compartment and a second compartment,   wherein the first port is in communication with the first compartment of the cavity, the first port located to a first side of the porous mesh,   wherein the second port is in communication with the second compartment of the cavity, the second port located to a second side of the porous mesh,   wherein the porous mesh is positioned within the cavity to have a substantially vertical orientation or an inclined orientation with respect to a flow of liquid through the porous mesh, and   wherein the cell separation device is configured for separating cells from microcarriers or spheroids in a liquid.   
     
     
         22 . The cell separation device of  claim 21 , wherein the viability of cells collected from the cell separation device are at least 98.1%. 
     
     
         23 . The cell separation device of  claim 21 , wherein the first port is located at one end of the vessel and the second port is located at an opposing end of the vessel. 
     
     
         24 . The cell separation device of  claim 21 , wherein the first port and the second port are off-set from one another on the vessel such that the porous mesh has the substantially vertical orientation within the cavity when (1) the first port is located in a substantially upward orientation and the second port is located in a substantially downward orientation, or (2) the first port is located in the substantially downward orientation and the second port is located in the substantially upward orientation. 
     
     
         25 . The cell separation device of  claim 21 , wherein the first port and the second port are in-line with one another on the vessel such that the porous mesh has the inclined orientation within the cavity when (1) the first port is located in a substantially upward orientation and the second port is located in a substantially downward orientation, or (2) the first port is located in the substantially downward orientation and the second port is located in the substantially upward orientation. 
     
     
         26 . The cell separation device of  claim 21 , wherein the first port and the second port are both positioned on one side of the vessel and the porous mesh has the substantially vertical orientation within the cavity when (1) the first port and the second port are located in a substantially upward orientation, or (2) the first port and the second port are located in a substantially downward orientation. 
     
     
         27 . The cell separation device of  claim 21 , wherein the porous mesh comprises pores therein with sizes ranging from about 12 microns to about 200 microns.