Insect resistant inir6 transgenic maize plants lacking a selectable marker and junction

Abstract

Transgenic INIR6 maize plants comprising modifications of the DP-4114 maize locus which provide for facile excision of the modified DP-4114 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.

1 . A transgenic maize plant cell comprising an INIR6 transgenic locus comprising the DNA molecule of SEQ ID NO: 34, the Cry1F, Cry34Ab1, and Cry35Ab1 genes of the DP-4114 transgenic locus and a deletion of both the 3′ DNA junction polynucleotide and phosphinotricin acetyltransferase (PAT) selectable marker gene of the DP-4114 transgenic locus. 
     
     
         2 . The transgenic maize plant cell of  claim 1 , wherein the INIR6 transgenic locus comprises the DNA molecule of SEQ ID NO: 25. 
     
     
         3 . (canceled) 
     
     
         4 . A transgenic maize plant part comprising the maize plant cell of  claim 1 . 
     
     
         5 . A The transgenic maize plant part of  claim 4 , wherein said maize plant part is a seed. 
     
     
         6 . A transgenic maize plant comprising the maize plant cell of  claim 1 . 
     
     
         7 . A method for obtaining a bulked population of inbred seed comprising selfing the transgenic maize plant of  claim 6  and harvesting seed comprising the INIR6 transgenic locus from the selfed maize plant. 
     
     
         8 . A method of obtaining hybrid maize seed comprising crossing the transgenic maize plant of  claim 6  to a second maize plant which is genetically distinct from the first maize plant and harvesting seed comprising the INIR6 transgenic locus from the cross. 
     
     
         9 .- 22 . (canceled)