US2024018194A1PendingUtilityA1

Insecticidal proteins and methods for their use

Assignee: PIONEER HI BRED INTPriority: Jan 15, 2015Filed: Aug 30, 2023Published: Jan 18, 2024

Est. expiryJan 15, 2035(~8.5 yrs left)· nominal 20-yr term from priority

Inventors:Steven D Gruver Heather Kozy Jessica O'Rear Barbara Rosen Ute Schellenberger Jun-Zhi Wei Weiping Xie Xiaohong Zhong Genhai Zhu

C07K 14/21C12N 15/8286A01N 37/46A01N 63/50Y02A40/146A01H 1/00A01N 63/27A01H 5/00C07H 21/04

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References

Claims

Abstract

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

Claims

exact text as granted — not AI-modified

That which is claimed: 
     
         1 . An insecticidal polypeptide selected from:
 a) a PIP-45-1 polypeptide comprising an amino acid sequence having greater than 80% sequence identity compared to the amino acid sequence of SEQ ID NO: 1;   b) a PIP-45-1 polypeptide comprising an amino acid sequence selected from SEQ ID NO: 1, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 39, SEQ ID NO: 43, SEQ ID NO: 45, SEQ ID NO: 234 and SEQ ID NO: 236;   c) a PIP-45-2 polypeptide comprising an amino acid sequence having greater than 80% sequence identity compared to the amino acid sequence of SEQ ID NO: 2;   d) a PIP-45-2 polypeptide comprises the amino acid sequence of SEQ ID NO: 2, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 28, SEQ ID NO: 30, SEQ ID NO: 32, SEQ ID NO: 34, SEQ ID NO: 36, SEQ ID NO: 40, SEQ ID NO: 44, SEQ ID NO: 46, SEQ ID NO: 235 or SEQ ID NO: 237;   e) a PIP-64-1 polypeptide comprising an amino acid sequence having greater than 80% sequence identity compared to SEQ ID NO: 53;   f) a PIP-64-1 polypeptide comprising the amino acid sequence of SEQ ID NO: 53, SEQ ID NO: 58 or SEQ ID NO: 238;   g) a PIP-64-2 polypeptide comprising an amino acid sequence having greater than 80% sequence identity compared to the amino acid sequence of SEQ ID NO: 54;   h) a PIP-64-2 polypeptide comprising the amino acid sequence of SEQ ID NO: 54, SEQ ID NO: 55, SEQ ID NO: 59 or SEQ ID NO: 239;   i) a PIP-74-1 polypeptide comprising an amino acid sequence having greater than 75% sequence identity compared to SEQ ID NO: 73;   j) a PIP-74-1 polypeptide comprising the amino acid sequence of SEQ ID NO: 73, SEQ ID NO: 75 or SEQ ID NO: 77;   k) a PIP-74-2 polypeptide comprising an amino acid sequence having greater than 80% sequence identity compared to the amino acid sequence of SEQ ID NO: 74;   l) a PIP-74-2 polypeptide comprising the amino acid sequence of SEQ ID NO: 74, SEQ ID NO: 76 or SEQ ID NO: 78;   m) a PIP-75 polypeptide comprising an amino acid sequence having greater than 80% sequence identity compared to the amino acid sequence of SEQ ID NO: 79;   n) a PIP-75 polypeptide comprising the amino acid sequence of SEQ ID NO: 79, SEQ ID NO: 80, SEQ ID NO: 81, SEQ ID NO: 84, SEQ ID NO: 85, SEQ ID NO: 86 or SEQ ID NO: 87;   o) a PIP-77 polypeptide comprising an amino acid sequence having greater than 80% sequence identity compared to the amino acid sequence of SEQ ID NO: 88; and   p) a PIP-77 polypeptide comprising the amino acid sequence of SEQ ID NO: 88, SEQ ID NO: 89, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO: 241, SEQ ID NO: 242 or SEQ ID NO: 245.   
     
     
         2 . An insecticidal composition comprising the PIP-45-1 polypeptide of  claim 1  and the PIP-45-2 polypeptide of  claim 1 . 
     
     
         3 . An insecticidal composition comprising the PIP-64-1 polypeptide of  claim 1 . 
     
     
         4 . An insecticidal composition comprising the PIP-64-1 polypeptide of  claim 1  and the PIP-64-2 polypeptide of claim. 
     
     
         5 . An insecticidal composition comprising the PIP-74-1 polypeptide of  claim 1  and the PIP-74-2 polypeptide of  claim 1 . 
     
     
         6 . An insecticidal composition comprising the PIP-75 polypeptide of  claim 1 . 
     
     
         7 . An insecticidal composition comprising the PIP-77 polypeptide of  claim 1 . 
     
     
         8 . A recombinant polynucleotide encoding an insecticidal polypeptide selected from:
 a) a PIP-45-1 polypeptide comprising an amino acid sequence having greater than 80% sequence identity compared to the amino acid sequence of SEQ ID NO: 1;   b) a PIP-45-1 polypeptide comprising an amino acid sequence selected from SEQ ID NO: 1, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 39, SEQ ID NO: 43, SEQ ID NO: 45, SEQ ID NO: 234 and SEQ ID NO: 236;   c) a PIP-45-2 polypeptide comprising an amino acid sequence having greater than 80% sequence identity compared to the amino acid sequence of SEQ ID NO: 2;   d) a PIP-45-2 polypeptide comprises the amino acid sequence of SEQ ID NO: 2, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 28, SEQ ID NO: 30, SEQ ID NO: 32, SEQ ID NO: 34, SEQ ID NO: 36, SEQ ID NO: 40, SEQ ID NO: 44, SEQ ID NO: 46, SEQ ID NO: 235 or SEQ ID NO: 237;   e) a PIP-64-1 polypeptide comprising an amino acid sequence having greater than 80% sequence identity compared to SEQ ID NO: 53;   f) a PIP-64-1 polypeptide comprising the amino acid sequence of SEQ ID NO: 53, SEQ ID NO: 58 or SEQ ID NO: 238;   g) a PIP-64-2 polypeptide comprising an amino acid sequence having greater than 80% sequence identity compared to the amino acid sequence of SEQ ID NO: 54;   h) a PIP-64-2 polypeptide comprising the amino acid sequence of SEQ ID NO: 54, SEQ ID NO: 55, SEQ ID NO: 59 or SEQ ID NO: 239;   i) a PIP-74-1 polypeptide comprising an amino acid sequence having greater than 75% sequence identity compared to SEQ ID NO: 73;   j) a PIP-74-1 polypeptide comprising the amino acid sequence of SEQ ID NO: 73, SEQ ID NO: 75 or SEQ ID NO: 77;   k) a PIP-74-2 polypeptide comprising an amino acid sequence having greater than 80% sequence identity compared to the amino acid sequence of SEQ ID NO: 74;   l) a PIP-74-2 polypeptide comprising the amino acid sequence of SEQ ID NO: 74, SEQ ID NO: 76 or SEQ ID NO: 78;   m) a PIP-75 polypeptide comprising an amino acid sequence having greater than 80% sequence identity compared to the amino acid sequence of SEQ ID NO: 79;   n) a PIP-75 polypeptide comprising the amino acid sequence of SEQ ID NO: 79, SEQ ID NO: 80, SEQ ID NO: 81, SEQ ID NO: 84, SEQ ID NO: 85, SEQ ID NO: 86 or SEQ ID NO: 87;   o) a PIP-77 polypeptide comprising an amino acid sequence having greater than 80% sequence identity compared to the amino acid sequence of SEQ ID NO: 88; and   p) a PIP-77 polypeptide comprising the amino acid sequence of SEQ ID NO: 88, SEQ ID NO: 89, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO: 241, SEQ ID NO: 242 or SEQ ID NO: 245.   
     
     
         9 . The recombinant polynucleotide of  claim 8 , wherein the recombinant polynucleotide is selected from:
 a) the polynucleotide of SEQ ID NO: 108, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: 142, SEQ ID NO: 146, SEQ ID NO: 150, SEQ ID NO: 152, SEQ ID NO: 220 or SEQ ID NO: 222;   b) the polynucleotide of SEQ ID NO: 109, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 147, SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 221 or SEQ ID NO: 223;   c) the polynucleotide SEQ ID NO: 160, SEQ ID NO: 165 or SEQ ID NO: 224;   d) the polynucleotide SEQ ID NO: 54, SEQ ID NO: 55, SEQ ID NO: 59 and SEQ ID NO: 239;   e) the polynucleotide SEQ ID NO: 180, SEQ ID NO: 182 and SEQ ID NO: 184;   f) the polynucleotide SEQ ID NO: 181, SEQ ID NO: 183 and SEQ ID NO: 185;   g) the polynucleotide SEQ ID NO: 186, SEQ ID NO: 187, SEQ ID NO: 188, SEQ ID NO: 191, SEQ ID NO: 192, SEQ ID NO: 193 and SEQ ID NO: 194; and   h) the polynucleotide SEQ ID NO: 195, SEQ ID NO: 196, SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID NO: 200, SEQ ID NO: 201, SEQ ID NO: 202, SEQ ID NO: 203, SEQ ID NO: 204, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 227, SEQ ID NO: 228 and SEQ ID NO: 231.   
     
     
         10 . A DNA construct comprising, the recombinant polynucleotide of  claim 8  and a heterologous regulatory sequence operably linked to the recombinant polynucleotide. 
     
     
         11 . A transgenic plant or plant cell comprising the DNA construct of  claim 10 . 
     
     
         12 . A method of inhibiting growth or killing an agricultural insect pest population, comprising contacting the insect pest population with an insecticidally-effective amount of the PIP-45-1 polypeptide of  claim 1  and the PIP-45-2 polypeptide of  claim 1 . 
     
     
         13 . A method of inhibiting growth or killing an agricultural insect pest, comprising contacting the insect pest with an insecticidally-effective amount of the PIP-64-1 polypeptide of  claim 1 . 
     
     
         14 . A method of inhibiting growth or killing an insect pest, comprising contacting the insect pest with an insecticidally-effective amount of the PIP-64-1 polypeptide of  claim 1  and the PIP-64-2 polypeptide of  claim 1 . 
     
     
         15 . A method of inhibiting growth or killing an insect pest, comprising contacting the insect pest with an insecticidally-effective amount of the PIP-74-1 polypeptide of  claim 1  and the PIP-74-2 polypeptide of  claim 1 . 
     
     
         16 . A method of inhibiting growth or killing an insect pest, comprising contacting the insect pest with an insecticidally-effective amount of the PIP-75 polypeptide of  claim 1 . 
     
     
         17 . A method of inhibiting growth or killing an insect pest, comprising contacting the insect pest with an insecticidally-effective amount of the PIP-77 polypeptide of  claim 1 . 
     
     
         18 . A method of controlling Lepidoptera and/or Coleoptera insect infestation in a transgenic plant and providing insect resistance management, comprising expressing in the plant the polynucleotide of  claim 8 . 
     
     
         19 . The method of  claim 12 , wherein the insect pest or insect pest population is resistant to a Bt toxin. 
     
     
         20 . Use of at least one insecticidal polypeptide of  claim 1  to inhibit growth or kill an insect or insect population.

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