Compositions and methods for treating cancer with duocars

Abstract

Novel therapeutic immunotherapy compositions comprising at least two vectors, each vector encoding a functional CAR, whereby the combination of vectors results in the expression of two or more non-identical binding domains, wherein each vector encoded binding domain(s) are covalently linked to a transmembrane domain and one or more non-identical intracellular signaling motifs are provided herein as well as are methods of use of same in a patient-specific immunotherapy that can be used to treat cancers and other diseases and conditions.

1 - 37 . (canceled) 
     
     
         38 . A method of treating a subject having an autoimmune disease, the method comprising administering to the subject having the autoimmune disease a pharmaceutical composition comprising an antitumor effective amount of a population of human T cells,
 wherein the population of human T-cells are autologous to the subject, and wherein each cell of the population of human T-cells comprises at least one multi-cistronic vector, each of the at least one multi-cistronic vector comprises a promoter operably linked to a multi-cistronic nucleic acid sequence encoding two or more functional CARs, wherein at least one of the two or more functional CARs comprises SEQ ID NO: 50, and thereby treating the subject having the autoimmune disease.   
     
     
         39 . The method of  claim 38 , wherein the population of human T-cells are infused directly into the subject. 
     
     
         40 . The method of  claim 38 , wherein the autoimmune disease is multiple sclerosis. 
     
     
         41 . The method of  claim 38 , wherein the autoimmune disease is a graft-versus-host disease. 
     
     
         42 . The method of  claim 38 , wherein the autoimmune disease is a skin allergy. 
     
     
         43 . The method of  claim 38 , wherein the autoimmune disease is a CD19+ autoimmune disease. 
     
     
         44 . The method of  claim 38 , wherein the population of human T-cells are T-regulatory cells. 
     
     
         45 . The method of  claim 38 , wherein the population of human T-cells are T helper 2 (Th-2 cells). 
     
     
         46 . The method of  claim 38 , wherein the population of human T-cells are T cells that promote a Th-2-like immune response.