Process for the Purification of L-Fucose from a Fermentation Broth

Abstract

The present invention concerns an efficient way to isolate L-fucose from a fermentation broth. The L-fucose contained in the fermentation broth is produced by microbial fermentation (bacterial or yeasts). The inventive process comprises a step of removing biomass from the fermentation broth, a step of subjecting the resulting solution to at least one of a cationic ion exchanger treatment and an anionic ion exchanger treatment and a step of removing salts after the ion exchanger treatment. The process can provide L-fucose in powder form, in granulated form as well as in form of L-fucose crystals.

1 .- 20 . (canceled) 
     
     
         21 . A food composition comprising L-fucose and at least one other carbohydrate selected from galactooligosaccharides (GOSs), fructooligosaccharides (FOSs), inulin, lactulose, isomaltose, maltodextrin, lactose, and human milk oligosaccharides (HMOs). 
     
     
         22 . The food composition according to  claim 21 , wherein the food composition comprises at least one human milk oligosaccharide selected from 2′-fucosyllactose, 3-fucosyllactose, lacto-N-tetraose, lacto-N-triose II, lacto-N-fucopentaose I, lacto-N-fucopentaose III, lacto-N-fucopentaose V, difucosyllactose, lacto-N-neotetraose, 3′-sialyllactose, 6′-sialyllactose, and sialylated lacto-N-neotetraose and lacto-N-tetraose derivatives. 
     
     
         23 . The food composition according to  claim 21 , wherein the food composition is
 i) a liquid food composition and comprises the L-fucose at a concentration of 1 mg/l to 2 g/l; or   ii) a solid food composition and comprises the L-fucose at a concentration of 5 mg/kg to 15 g/kg.   
     
     
         24 . The food composition according to  claim 21 , wherein the food composition comprises
 i) at least one neutral human milk oligosaccharide; and   ii) at least one acidic human milk oligosaccharide.   
     
     
         25 . The food composition according to  claim 21 , wherein the food composition comprises at least one substance selected from lactose, whey protein, biotin, skimmed milk, vegetable oil, skimmed milk powder, oil of  Mortierella  alpine, fish oil, calcium carbonate, potassium chloride, vitamin C, sodium chloride, vitamin E, iron acetate, zinc sulfate, niacin, calcium-D-panthothenate, copper sulfate, vitamin A, vitamin B1, vitamin B6, magnesium sulphate, potassium iodate, folic acid, vitamin K, sodium selenite and vitamin D. 
     
     
         26 . The food composition according to  claim 21 , wherein the food composition comprises at least one substance selected from a protein source, a vitamin, an oil, a mineral, an enzyme, a further carbohydrate, and a probiotic strain. 
     
     
         27 . The food composition according to  claim 21 , wherein the food composition is selected from a medical food composition, a dietary supplement, a sachet product, a liquid ready-to-use infant nutrition product, a liquid ready-to-use toddler nutrition product, a granulated product, a spray-dried infant formula product, a premix thereof, and combinations thereof. 
     
     
         28 . The food composition according to  claim 27 , wherein the food composition is a liquid, ready-to-use infant nutrition product or a liquid-ready-to-use toddler nutrition product comprising L-fucose at a concentration of 1 mg/l to 2 g/l, a sialic acid and
 i) at least one neutral human milk oligosaccharide selected from 2′-fucosyllactose, 3-fucosyllactose, difucosyllactose, lacto-N-tetraose, and lacto-N-neotetraose; and/or   ii) at least one acidic human milk oligosaccharide selected from 3′-sialylactose and 6′-sialyllactose.   
     
     
         29 . The food composition according to  claim 27 , wherein the food composition is a spray-dried infant formula product comprising L-fucose at a concentration of 5 mg/kg to 15 g/kg, a sialic acid and
 i) at least one neutral human milk oligosaccharide selected from 2′-fucosyllactose, 3-fucosyllactose, difucosyllactose, lacto-N-tetraose and lacto-N-neotetraose; and/or   ii) at least one acidic human milk oligosaccharide selected from 3′-sialyllactose and 6′-sialyllactose.   
     
     
         30 . The food composition according to  claim 27 , wherein the food composition is a dietary supplement comprising L-fucose and human milk oligosaccharides, wherein the human milk oligosaccharides comprise at least one neutral HMO selected from 2′-fucosyllactose, 3-fucosyllactose, difucosyllactose, lacto-N-triose II, lacto-N-tetraose and lacto-N-neotetraose, and lacto-N-fucopentaose I. 
     
     
         31 . The food composition according to  claim 27 , wherein the food composition is a premix further comprising at least one substance selected from a protein source, a vitamin, an oil, a mineral, an enzyme, a further carbohydrate, and a probiotic strain. 
     
     
         32 . (canceled) 
     
     
         33 . (canceled) 
     
     
         34 . The food composition according to  claim 24 , wherein the at least one neutral human milk oligosaccharide is selected from 2′-fucosyllactose, 3-fucosyllactose, lacto-N-tetraose, lacto-N-neotetraose, and combinations thereof. 
     
     
         35 . The food composition according to  claim 24 , wherein the at least one acidic human milk oligosaccharide is selected from 3′-sialyllactose, 6′-sialyllactose, and combinations thereof. 
     
     
         36 . The food composition according to  claim 24 , wherein the sialic acid is N-acetylneuraminic acid. 
     
     
         37 . The food composition according to  claim 31 , wherein the protein source is selected from whey, corn soya blend, protein hydrolysates, and combinations thereof. 
     
     
         38 . The food composition according to  claim 31 , wherein the vitamin is selected from vitamin A, thiamine, riboflavin, vitamin B12, folate, and combinations thereof. 
     
     
         39 . The food composition according to  claim 31 , wherein the oil is selected from palm oil, docosahexaenoic acid (DHA), arachidonic acid (ARA), and combinations thereof. 
     
     
         40 . The food composition according to  claim 31 , wherein the enzyme is selected from amylase, amyloglucosidase, and combinations thereof. 
     
     
         41 . The food composition according to  claim 31 , wherein the further carbohydrate is selected from a human milk oligosaccharide (HMO), a galactooligosaccharide (GOS), inulin, fructooligosaccharides (FOS), lactose, isomaltose, sialic acid, and combinations thereof. 
     
     
         42 . The food composition according to  claim 31 , wherein the probiotic strain is selected from  Lactobacillus, Bifidobacterium, Bacillus , yeast, and combinations thereof.