Malassezia factor with antibiotic activity

Abstract

Compositions including an antibiotic factor from Malassezia are provided. In some examples, the compositions include a Malassezia exoproduct, a Malassezia cell-free supernatant, or a Malassezia cell and a pharmaceutically acceptable carrier. Methods of treating an infection in a subject, such as a Staphylococcus infection are also provided.

We claim: 
     
         1 . A composition comprising a  Malassezia  exoproduct, a  Malassezia  cell-free supernatant, or a live  Malassezia  cell; and a pharmaceutically acceptable carrier. 
     
     
         2 . The composition of  claim 1 , wherein the composition comprises the  Malassezia  exoproduct. 
     
     
         3 . The composition of  claim 1 , wherein the composition comprises the  Malassezia  cell-free supernatant. 
     
     
         4 . The composition of  claim 1 , wherein the composition comprises the live  Malassezia  cell. 
     
     
         5 . The composition of  claim 1 , wherein the  Malassezia  comprises  Malassezia dermatis, Malassezia furfur, Malassezia globosa, Malassezia pachydermatis, Malassezia restricta , or  Malassezia sympodialis.    
     
     
         6 . The composition of  claim 5 , wherein the  Malassezia  comprises  Malassezia sympodialis.    
     
     
         7 . The composition of  claim 1 , wherein the composition inhibits growth of a  Staphylococcus  species by at least 10-fold compared to a control. 
     
     
         8 . The composition of  claim 7 , wherein the  Staphylococcus  species is  S. aureus , methicillin-resistant  S. aureus  (MRSA), vancomycin-resistant  S. aureus  (VRSA),  S. caprae, S. epidermidis, S. haemolyticus , or  S. warneri.    
     
     
         9 . The composition of  claim 1 , wherein the  Malassezia  exoproduct exhibits one or more of heat resistance, binding to non-polar molecules, and pH sensitivity. 
     
     
         10 . The composition of  claim 9 , wherein the pH sensitivity comprises antimicrobial activity at pH of about 6 or less. 
     
     
         11 . The composition of  claim 10 , wherein the composition has a pH of about 6 or less. 
     
     
         12 . The composition of  claim 11 , wherein the composition has a pH of about 5.4 to about 6. 
     
     
         13 . The composition of  claim 1 , wherein the composition comprises about 0.01%-10% w/w, 0.01-10% v/v, or 0.01-10% w/v of the  Malassezia  exoproduct,  Malassezia  cell free supernatant, or live  Malassezia  cells. 
     
     
         14 . The composition of  claim 1 , further comprising an additional antibiotic compound. 
     
     
         15 . The composition of  claim 1 , wherein the composition is formulated for topical administration. 
     
     
         16 . The composition of  claim 15 , wherein the topical formulation comprises a solution, a gel, an ointment, a cream, a lotion, a paste, or a suspension. 
     
     
         17 . The composition of  claim 15 , wherein the pharmaceutically acceptable carrier is a base comprising a plurality of inactive ingredients. 
     
     
         18 . A composition comprising a  Malassezia  exoproduct, a  Malassezia  cell-free supernatant, or a  Malassezia  cell; and a pharmaceutically acceptable carrier, wherein the composition is formulated for topical administration. 
     
     
         19 . The composition of  claim 18 , wherein the topical formulation comprises a gel, an ointment, a cream, a lotion, or a paste. 
     
     
         20 . The composition of  claim 18 , wherein the topical formulation comprises an alcohol or a fragrance.