Plant regulatory elements and uses thereof

Abstract

The invention provides DNA molecules and constructs, and their nucleotide sequences, useful for modulating gene expression in plants, and for specifying intracellular or extracellular localization of a gene product of interest. Transgenic plants, plant cells, plant parts, and seeds, comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided.

1 . A DNA molecule comprising a DNA sequence selected from the group consisting of:
 a) a sequence with at least 85 percent sequence identity to SEQ ID NO:233;   b) a sequence comprising SEQ ID NO:233; and   c) a fragment of SEQ ID NO:233, wherein the fragment has gene-regulatory activity;   wherein said sequence is operably linked to a heterologous transcribable polynucleotide molecule.   
     
     
         2 . The DNA molecule of  claim 1 , wherein said sequence has at least 90 percent sequence identity to the DNA sequence of SEQ ID NO:233. 
     
     
         3 . The DNA molecule of  claim 1 , wherein said sequence has at least 95 percent sequence identity to the DNA sequence of SEQ ID NO:233. 
     
     
         4 . The DNA molecule of  claim 1 , wherein the DNA sequence comprises a regulatory element. 
     
     
         5 . The DNA molecule of  claim 1 , wherein the heterologous transcribable polynucleotide molecule comprises a gene of agronomic interest. 
     
     
         6 . The DNA molecule of  claim 1 , wherein the heterologous transcribable polynucleotide molecule comprises a gene capable of providing herbicide resistance in plants. 
     
     
         7 . The DNA molecule of  claim 1 , wherein the transcribable polynucleotide molecule comprises a gene capable of providing plant pest control in plants. 
     
     
         8 . A transgenic plant cell comprising a DNA construct comprising a sequence selected from the group consisting selected from the group consisting of:
 a) a sequence with at least 85 percent sequence identity to SEQ ID NO:233;   b) a sequence comprising SEQ ID NO:233; and   c) a fragment of SEQ ID NO:233, wherein the fragment has gene-regulatory activity;   wherein said sequence is operably linked to a heterologous transcribable polynucleotide molecule.   
     
     
         9 . The transgenic plant cell of  claim 8 , wherein said transgenic plant cell is a monocotyledonous plant cell. 
     
     
         10 . The transgenic plant cell of  claim 8 , wherein said transgenic plant cell is a dicotyledonous plant cell. 
     
     
         11 . A transgenic plant, or part thereof, comprising the DNA molecule of  claim 1 . 
     
     
         12 . A progeny plant of the transgenic plant of  claim 11 , or a part thereof, wherein the progeny plant or part thereof comprises said DNA molecule. 
     
     
         13 . A transgenic seed, wherein the seed comprises the DNA molecule of  claim 1 . 
     
     
         14 .- 21 . (canceled)