US2024188520A1PendingUtilityA1

Method for identifying whether a diploid potato is self-compatible

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Assignee: UNIV YUNNANPriority: Dec 4, 2019Filed: Feb 8, 2024Published: Jun 13, 2024
Est. expiryDec 4, 2039(~13.4 yrs left)· nominal 20-yr term from priority
Inventors:Ling MaYi ShangSanwen HuangChunzhi ZhangDongli Gao
A01H 5/06A01H 1/045G16B 30/00A01H 6/827C12Q 2600/158C12Q 2600/156C12Q 2600/13C12Q 1/6895C12Q 1/6858C07K 14/415A01H 5/04A01H 1/022C12Q 1/6809
47
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Claims

Abstract

The present application relates to the technical field of genetic breeding, and provides a method for identifying whether a diploid potato is self-compatible. The method relates to identifying whether a StSCI gene in the diploid potato is transcribed and expressed. Also disclosed is a method for identifying whether a StSCI gene is expressed by using molecular marker, and a method of screening for the molecular marker, which includes: obtaining the genome sequence information of parental materials, screening for difference sites of the parental materials, screening for the molecular marker, and identifying whether the screened molecular marker are usable. As for the identification of the self-compatibility of a diploid potato by using the screened molecular marker, the identification workload is small, a lot of time is saved, and the identification result is not affected by the environment, and it is accurate and reliable.

Claims

exact text as granted — not AI-modified
What is claimed is: 
     
         1 . A diploid self-compatible potato plant or part thereof, comprising (i) a genomic locus comprising a sequence having 90% or more identity to SEQ ID NO: 1 or 2 and (ii) the T allele of a single nucleotide polymorphism (SNP) identifiable via KASP marker primers comprising sequences set forth in SEQ ID Nos: 3 and 4, wherein the diploid self-compatible potato plant is not RH89-039-16 or progeny thereof. 
     
     
         2 . The diploid self-compatible potato plant or part thereof of  claim 1 , wherein the genomic locus comprising a sequence having 95% or more identity to SEQ ID NO: 1 or 2. 
     
     
         3 . The diploid self-compatible potato plant or part thereof of  claim 1 , wherein the genomic locus comprising a sequence having 98% or more identity to SEQ ID NO: 1 or 2. 
     
     
         4 . The diploid self-compatible potato plant or part thereof of  claim 1 , wherein the genomic locus comprising a sequence having 100% identity to SEQ ID NO: 1 or 2. 
     
     
         5 . The diploid self-compatible potato plant or part thereof of  claim 1 , wherein the part thereof is selected from the group consisting of seed, fruit, leaf, and flower. 
     
     
         6 . The diploid self-compatible potato plant or part thereof of  claim 1 , wherein the diploid self-compatible potato plant is homozygous for the genomic locus. 
     
     
         7 . The diploid self-compatible potato plant or part thereof of  claim 1 , wherein the diploid self-compatible potato plant is a hybrid. 
     
     
         8 . A diploid self-compatible potato plant or part thereof, comprising (i) a homozygous genomic locus comprising a sequence having 90% or more identity to SEQ ID NO: 1 or 2 and (ii) the T allele of a single nucleotide polymorphism (SNP) marker identifiable via KASP primers comprising sequences set forth in SEQ ID Nos: 3 and 4. 
     
     
         9 . The diploid self-compatible potato plant or part thereof of  claim 8 , wherein the genomic locus comprising a sequence having 95% or more identity to SEQ ID NO: 1 or 2. 
     
     
         10 . The diploid self-compatible potato plant or part thereof of  claim 8 , wherein the genomic locus comprising a sequence having 98% or more identity to SEQ ID NO: 1 or 2. 
     
     
         11 . The diploid self-compatible potato plant or part thereof of  claim 8 , wherein the genomic locus comprising a sequence having 100% identity to SEQ ID NO: 1 or 2. 
     
     
         12 . The diploid self-compatible potato plant or part thereof of  claim 8 , wherein the part thereof is selected from the group consisting of seed, fruit, leaf, and flower. 
     
     
         13 . The diploid self-compatible potato plant or part thereof of  claim 8 , wherein the diploid self-compatible potato plant is homozygous for the T allele of the SNP marker. 
     
     
         14 . The diploid self-compatible potato plant or part thereof of  claim 8 , wherein the diploid self-compatible potato plant is not RH89-039-16 or progeny thereof. 
     
     
         15 . The diploid self-compatible potato plant or part thereof of  claim 8 , wherein the diploid self-compatible potato plant is a hybrid. 
     
     
         16 . A diploid self-compatible potato plant or part thereof, comprising (i) a genomic locus comprising a sequence having 90% or more identity to SEQ ID NO: 1 or 2 and (ii) the 11 bp deletion self-compatible allele of an InDel molecular marker identifiable via primers comprising sequences set forth in SEQ ID Nos: 5 and 6, wherein the diploid self-compatible potato plant is not RH89-039-16 or progeny thereof. 
     
     
         17 . The diploid self-compatible potato plant or part thereof of  claim 16 , wherein the genomic locus comprising a sequence having 95% or more identity to SEQ ID NO: 1 or 2. 
     
     
         18 . The diploid self-compatible potato plant or part thereof of  claim 16  wherein the genomic locus comprising a sequence having 98% or more identity to SEQ ID NO: 1 or 2. 
     
     
         19 . The diploid self-compatible potato plant or part thereof of  claim 16 , wherein the genomic locus comprising a sequence having 100% identity to SEQ ID NO: 1 or 2. 
     
     
         20 . The diploid self-compatible potato plant or part thereof of  claim 16 , wherein the part thereof is selected from the group consisting of seed, fruit, leaf, and flower. 
     
     
         21 . The diploid self-compatible potato plant or part thereof of  claim 16 , wherein the diploid self-compatible potato plant is homozygous for the genomic locus. 
     
     
         22 . The diploid self-compatible potato plant or part thereof of  claim 16 , wherein the diploid self-compatible potato plant is a hybrid. 
     
     
         23 . A diploid self-compatible potato plant or part thereof, comprising (i) a homozygous genomic locus comprising a sequence having 90% or more identity to SEQ ID NO: 1 or 2 and (ii) the 11 bp deletion self-compatible allele of an InDel molecular marker identifiable via primers comprising sequences set forth in SEQ ID Nos: 5 and 6. 
     
     
         24 . The diploid self-compatible potato plant or part thereof of  claim 23 , wherein the genomic locus comprising a sequence having 95% or more identity to SEQ ID NO: 1 or 2. 
     
     
         25 . The diploid self-compatible potato plant or part thereof of  claim 23 , wherein the genomic locus comprising a sequence having 98% or more identity to SEQ ID NO: 1 or 2. 
     
     
         26 . The diploid self-compatible potato plant or part thereof of  claim 23 , wherein the genomic locus comprising a sequence having 100% identity to SEQ ID NO: 1 or 2. 
     
     
         27 . The diploid self-compatible potato plant or part thereof of  claim 23 , wherein the part thereof is selected from the group consisting of seed, fruit, leaf, and flower. 
     
     
         28 . The diploid self-compatible potato plant or part thereof of  claim 23 , wherein the plant is homozygous for the 11 bp deletion self-compatible allele of the InDel molecular marker. 
     
     
         29 . The diploid self-compatible potato plant or part thereof of  claim 23 , wherein the diploid self-compatible potato plant is not RH89-039-16 or progeny thereof. 
     
     
         30 . The diploid self-compatible potato plant or part thereof of  claim 23 , wherein the diploid self-compatible potato plant is a hybrid.

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