Nomadic bacteria and uses thereof

Abstract

A method of generating a biofilm of nomadic bacteria. The method comprises:(a) culturing the nomadic bacteria in an acidic environment under conditions that promote generation of a V-type structure of the nomadic bacteria; and subsequently(b) culturing said nomadic bacteria having a V-type structure on an adherent surface, thereby generating the biofilm comprising the nomadic bacteria. Conditioned media of nomadic bacteria are also disclosed and uses thereof.

What is claimed is: 
     
         1 . A method of generating a biofilm comprising a nomadic bacteria, the method comprising:
 (a) culturing the nomadic bacteria in an acidic environment under conditions that promote generation of a V-type structure of the nomadic bacteria; and subsequently   (b) culturing said nomadic bacteria having a V-type structure on an adherent surface, thereby generating the biofilm comprising the nomadic bacteria.   
     
     
         2 . The method of  claim 1 , wherein said nomadic bacteria are of the  Lactobacillus  genus. 
     
     
         3 . The method of  claim 2 , wherein said nomadic bacteria are of a species selected from the group consisting of  L. rhamnosus, L. plantarum  and  L. casei.    
     
     
         4 . The method of  claim 3 , wherein said nomadic bacteria is of the  L. plantarum  species and said acidic environment is between pH 2.5-pH 4 and/or said nomadic bacteria is of the  L. casei  species and said acidic environment is between pH 3.5-pH 6.5. 
     
     
         5 . The method of  claim 1 , further comprising culturing an additional bacteria on said adherent surface so as to generate a biofilm comprising the nomadic bacteria and said additional bacteria. 
     
     
         6 . The method of  claim 5 , wherein said additional bacteria comprises  Bacillus subtilis.    
     
     
         7 . A biofilm generated according to the method of  claim 1 . 
     
     
         8 . A composition comprising isolated, nomadic bacteria, wherein at least 30% of the bacteria have a V-shaped structure. 
     
     
         9 . The composition of  claim 8 , wherein said isolated, nomadic bacteria are of the  Lactobacillus  genus. 
     
     
         10 . The composition of  claim 9 , wherein said isolated, nomadic bacteria are of a species selected from the group consisting of  L. rhamnosus, L. plantarum  and  L. casei.    
     
     
         11 . A method of reducing biofilm formation of a pathogen comprising:
 (a) culturing a nomadic bacteria in a medium subjected to at least one stress selected from the group consisting of a pH stress, a temperature stress, an oxidative stress, an osmotic stress and a combination thereof to generate a conditioned medium; and   (b) contacting said pathogen with said conditioned medium under conditions that reduce biofilm formation of said pathogen, thereby reducing biofilm formation of the pathogen.   
     
     
         12 . The method of  claim 11 , wherein said combination comprises an acidic stress and a cold stress. 
     
     
         13 . The method of  claim 11 , wherein said culturing promotes a chain structure of said nomadic bacteria. 
     
     
         14 . The method of  claim 11 , wherein said pathogen is a pathogenic bacteria or a fungus. 
     
     
         15 . The method of  claim 11 , wherein said nomadic bacteria are of a species selected from the group consisting of  L. rhamnosus, L. plantarum  and  L. casei.    
     
     
         16 . A conditioned medium generated by culturing nomadic bacteria in a medium subjected to at least one stress selected from the group consisting of a pH stress, a temperature stress, an oxidative stress, an osmotic stress and a combination thereof. 
     
     
         17 . The conditioned medium of  claim 16 , wherein said culturing is effected on a non-adherent surface. 
     
     
         18 . The conditioned medium of  claim 16 , wherein said nomadic bacteria are of a species selected from the group consisting of  L. rhamnosus, L. plantarum  and  L. casei.    
     
     
         19 . The conditioned medium of  claim 16 , wherein said at least one stress comprises an acid stress and/or a cold stress. 
     
     
         20 . The conditioned medium of  claim 16 , comprising 2-undecanone.