Natural water-soluble plant compound with antidepressant and sleep-promoting effects, and preparation method and use thereof

Abstract

The present disclosure relates to a natural water-soluble plant compound with antidepressant and sleep-promoting effects, and a preparation method and use thereof. In the present disclosure, the preparation method includes: immersing Fructus lycii, green tea, spina date seed, lily, sugarcane, peanut stem, Euglena, and Chlorella in water, and conducting ultrasonication and first solid-liquid separation to obtain a plant extract; mixing the plant extract with an organic extractant to extract an extracted aqueous phase; removing a solvent of the extracted aqueous phase to obtain a crude extract; dispersing the crude extract into water, and conducting second solid-liquid separation to obtain a purified water extract; and drying the purified water extract to obtain the natural water-soluble plant compound. The natural water-soluble plant compound shows a wide range of neuroactivity, and has an important broad-spectrum improvement effect on alleviating various discomforts caused by the fast-paced and high-stress life of modern people.

1 . A preparation method of a natural water-soluble plant compound, comprising the following steps:
 immersing a plant raw material in water, and conducting ultrasonication and first solid-liquid separation to obtain a plant extract; wherein the plant raw material comprises  Fructus lycii , green tea, spina date seed, lily, sugarcane, peanut stem,  Euglena , and  Chlorella;      mixing the plant extract with an organic extractant to extract an extracted aqueous phase;   removing a solvent of the extracted aqueous phase to obtain a crude extract;   dispersing the crude extract into water, and conducting second solid-liquid separation to obtain a purified water extract; and   drying the purified water extract to obtain the natural water-soluble plant compound.   
     
     
         2 . The preparation method according to  claim 1 , wherein the plant raw material comprises the following components in parts by mass:
 20 parts to 50 parts of the  Fructus lycii,  15 parts to 40 parts of the green tea, 15 parts to 30 parts of the spina date seed, 10 parts to 20 parts of the lily, 5 parts to 20 parts of the sugarcane, 3 parts to 10 parts of the peanut stem, 1 part to 10 parts of the  Euglena , and 1 part to 10 parts of the  Chlorella.      
     
     
         3 . The preparation method according to  claim 1 , wherein the organic extractant is ethyl acetate; and the plant extract and the organic extractant are at a volume ratio of 1:(3-4). 
     
     
         4 . The preparation method according to  claim 1 , wherein the solvent is removed by rotary evaporation at 60° C. to 65° C. and 9 rpm to 10 rpm. 
     
     
         5 . The preparation method according to  claim 1 , wherein the ultrasonication is conducted in an ice-water bath at an ultrasonic power of 700 W to 1,000 W for 8 min to 10 min. 
     
     
         6 . The preparation method according to  claim 1 , wherein the drying refers to freeze-drying at −80° C. to −70° C. for 30 min to 40 min. 
     
     
         7 . The preparation method according to  claim 1 , wherein the first solid-liquid separation comprises first centrifugal separation and membrane filtration that are sequentially conducted;
 the first centrifugal separation is conducted at a rotational speed of 4,000 g to 5,000 g for 5 min to 8 min; and the membrane filtration is conducted using a filter membrane with a pore size of 0.22 μm; and   the second solid-liquid separation is conducted by second centrifugal separation at a rotational speed of 8,000 g to 10,000 g for 10 min.   
     
     
         8 . A natural water-soluble plant compound prepared by the preparation method according to  claim 1 , comprising y-aminobutyric acid, phenylacetic acid, glutamic acid, glycine, and taurine. 
     
     
         9 . The natural water-soluble plant compound according to  claim 8 , wherein the plant raw material comprises the following components in parts by mass:
 20 parts to 50 parts of the  Fructus lycii,  15 parts to 40 parts of the green tea, 15 parts to 30 parts of the spina date seed, 10 parts to 20 parts of the lily, 5 parts to 20 parts of the sugarcane, 3 parts to 10 parts of the peanut stem, 1 part to 10 parts of the  Euglena , and 1 part to 10 parts of the  Chlorella.      
     
     
         10 . The natural water-soluble plant compound according to  claim 8 , wherein the organic extractant is ethyl acetate; and the plant extract and the organic extractant are at a volume ratio of 1:(3-4). 
     
     
         11 . The natural water-soluble plant compound according to  claim 8 , wherein the solvent is removed by rotary evaporation at 60° C. to 65° C. and 9 rpm to 10 rpm. 
     
     
         12 . The natural water-soluble plant compound according to  claim 8 , wherein the ultrasonication is conducted in an ice-water bath at an ultrasonic power of 700 W to 1,000 W for 8 min to 10 min. 
     
     
         13 . The natural water-soluble plant compound according to  claim 8 , wherein the drying refers to freeze-drying at −80° C. to −70° C. for 30 min to 40 min. 
     
     
         14 . The natural water-soluble plant compound according to  claim 8 , wherein the first solid-liquid separation comprises first centrifugal separation and membrane filtration that are sequentially conducted;
 the first centrifugal separation is conducted at a rotational speed of 4,000 g to 5,000 g for 5 min to 8 min; and the membrane filtration is conducted using a filter membrane with a pore size of 0.22 μm; and   the second solid-liquid separation is conducted by second centrifugal separation at a rotational speed of 8,000 g to 10,000 g for 10 min.   
     
     
         15 . The natural water-soluble plant compound according to  claim 8 , comprising (1-200) μg/100 mg of the y-aminobutyric acid, (0.1-50) μg/100 mg of the phenylacetic acid, (0.1-20) μg/100 mg of the glutamic acid, (0.01-8) μg/100 mg of the glycine, and (0.01-0.2) μg/100 mg of the taurine. 
     
     
         16 . The natural water-soluble plant compound according to  claim 9 , comprising (1-200) μg/100 mg of the y-aminobutyric acid, (0.1-50) μg/100 mg of the phenylacetic acid, (0.1-20) μg/100 mg of the glutamic acid, (0.01-8) μg/100 mg of the glycine, and (0.01-0.2) μg/100 mg of the taurine. 
     
     
         17 . The natural water-soluble plant compound according to  claim 10 , comprising (1-200) μg/100 mg of the y-aminobutyric acid, (0.1-50) μg/100 mg of the phenylacetic acid, (0.1-20) μg/100 mg of the glutamic acid, (0.01-8) μg/100 mg of the glycine, and (0.01-0.2) μg/100 mg of the taurine. 
     
     
         18 . The natural water-soluble plant compound according to  claim 11 , comprising (1-200) μg/100 mg of the y-aminobutyric acid, (0.1-50) μg/100 mg of the phenylacetic acid, (0.1-20 μg/100 mg of the glutamic acid, (0.01-8) μg/100 mg of the glycine, and (0.01-0.2) μg/100 mg of the taurine. 
     
     
         19 . The natural water-soluble plant compound according to  claim 12 , comprising (1-200) μg/100 mg of the y-aminobutyric acid, (0.1-50) μg/100 mg of the phenylacetic acid, (0.1-20) μg/100 mg of the glutamic acid, (0.01-8) μg/100 mg of the glycine, and (0.01-0.2) μg/100 mg of the taurine. 
     
     
         20 . A drug or a health care product for treating insomnia and/or depression, comprising the natural water-soluble plant compound according to  claim 8 .