Microfluidic chip and microfluidic chip detection system

Abstract

The present disclosure relates to a microfluidic chip and a microfluidic chip detection system. The microfluidic chip including: a storage member provided with a groove therein, at least two storage chambers being disposed around the groove; a base disposed at one end of the storage member away from the groove, a reaction chamber being disposed on the base; and a valve disposed in the groove, the valve being configured to operatively communicate any one of the at least two storage chambers with the reaction chamber. The present disclosure can lead the solution in any storage chamber to the reaction chamber or lead the solution in the reaction chamber to any storage chamber by operating the valve, so that the solution transfer is realized, the structure is simple and compact, the length of the flow channels can be greatly shortened, and the detection efficiency is improved.

1 . A microfluidic chip, comprising:
 a storage member provided with a groove therein, at least two storage chambers being disposed around the groove;   a base disposed at one end of the storage member away from the groove, a reaction chamber being disposed on the base; and   a valve disposed in the groove, the valve being configured to operatively communicate any one of the at least two storage chambers with the reaction chamber.   
     
     
         2 . The microfluidic chip according to  claim 1 , wherein at least two first storage member inner flow channels are disposed in the storage member, each first storage member inner flow channel is correspondingly communicated with one storage chamber, a valve inner flow channel communicated with the reaction chamber is disposed in the valve, and the valve is configured to operatively communicate the valve inner flow channel with any first storage member inner flow channel. 
     
     
         3 . The microfluidic chip according to  claim 2 , wherein a first end of each first storage member inner flow channel penetrates a bottom wall of the groove, the valve is configured to operatively communicate the valve inner flow channel with the first end of the first storage member inner flow channel, and a second end of each first storage member inner flow channel is communicated with the storage chamber via one side of the storage chamber adjacent to the base. 
     
     
         4 . The microfluidic chip according to  claim 3 , wherein the second ends of the first storage member inner flow channels are communicated with parts of the storage chambers at the lowest positions. 
     
     
         5 . The microfluidic chip according to  claim 3 , wherein both a first end and a second end of the valve inner flow channel penetrate one end of the valve adjacent to the bottom wall of the groove, the first end of the valve inner flow channel is communicated with the reaction chamber, the second end of the valve inner flow channel is operatively communicated with any first storage member inner flow channel, the first end of the valve inner flow channel is located in a middle of the valve, and the second end of the valve inner flow channel is close to an outer edge of the valve. 
     
     
         6 . The microfluidic chip according to  claim 1 , wherein the valve comprises:
 a rotor rotatably disposed in the groove, the rotor comprises a valve seat and a valve stem, and the valve stem being connected with the valve seat; and   a valve cover connected with a circumferential side wall of the groove and abutting against the valve seat to limit the valve seat between the valve cover and the bottom wall of the groove, the valve cover being provided with a first through hole, an operating part of the valve stem penetrating the first through hole, and the operating part of the valve stem being configured to be connected with an external operating member.   
     
     
         7 . (canceled) 
     
     
         8 . The microfluidic chip according to  claim 1 , further comprising: a sealing film, the at least two storage chambers comprise a reagent chamber, the sealing film is configured to seal the reagent chamber, the microfluidic chip further comprises a top cover and a puncture needle, the top cover is disposed at one end of the storage member provided with the groove, the puncture needle is connected to the top cover, and the puncture needle is configured to press against the sealing film under the action of an external force to puncture the sealing film. 
     
     
         9 . The microfluidic chip according to  claim 8 , wherein the top cover comprises a first rib, the puncture needle is connected to the first rib, and the first rib is configured to be disconnected under the action of the external force, so that the puncture needle is separated from the top cover and pressed against the sealing film. 
     
     
         10 . The microfluidic chip according to  claim 8 , wherein a middle of the top cover is provided with a second through hole, and the second through hole is configured to allow the external operating member to penetrate to operate the valve. 
     
     
         11 . The microfluidic chip according to  claim 8 , wherein the puncture needle is provided with a needle inner gas channel therein, and a third through hole communicating an outer part of the puncture needle with the needle inner gas channel is disposed near a part of the puncture needle connected with the top cover. 
     
     
         12 . The microfluidic chip according to  claim 11 , further comprising a cover sheet, the cover sheet is disposed in the top cover, the cover sheet is provided with a fourth through hole allowing the puncture needle to penetrate, and the puncture needle is configured to press against the sealing film under the action of the external force, and continue to press against the sealing film after puncturing the sealing film, so that the third through hole is sealed by the cover sheet. 
     
     
         13 . The microfluidic chip according to  claim 1 , wherein the reaction chamber protrudes towards one side away from the storage member. 
     
     
         14 . (canceled) 
     
     
         15 . The microfluidic chip according to  claim 1 , further comprising an amplification member, the amplification member is provided with an amplification chamber, a side part of the storage member is provided with a slot, the slot is located between two adjacent storage chambers, the amplification member is connected with the slot in an inserting way, and the valve is configured to operatively communicate the reaction chamber with the amplification chamber. 
     
     
         16 . The microfluidic chip according to  claim 15 , wherein the storage member is provided with a second storage member inner flow channel, a first end of the second storage member inner flow channel penetrates the slot, a second end of the second storage member inner flow channel penetrates the groove, the amplification member is provided with an amplification member inner flow channel communicated with the amplification chamber, the amplification member inner flow channel is communicated with the first end of the second storage member inner flow channel, and the valve is configured to be operatively communicated with the second end of the second storage member inner flow channel to guide a solution in the reaction chamber to the amplification chamber through the second storage member inner flow channel and the amplification member inner flow channel. 
     
     
         17 . The microfluidic chip according to  claim 16 , wherein the storage member is provided with a third storage member inner flow channel, a first end of the third storage member inner flow channel penetrates the slot, a second end of the third storage member inner flow channel penetrates the groove, the amplification member is provided with an amplification member inner gas channel communicated with the amplification chamber, the amplification member inner gas channel is communicated with the first end of the third storage member inner flow channel, and the valve is configured to be operatively communicated with the second end of the third storage member inner flow channel to guide gas in the amplification chamber to one storage chamber through the amplification member inner gas channel and the third storage member inner flow channel. 
     
     
         18 . The microfluidic chip according to  claim 15 , wherein the valve inner flow channel communicated with the reaction chamber is disposed in the valve, a valve inner gas channel is also disposed in the valve, and the valve is configured to operatively communicate the valve inner flow channel with the reaction chamber and the amplification chamber, and communicate the valve inner gas channel with the amplification chamber and one storage chamber. 
     
     
         19 . The microfluidic chip according to  claim 1 , wherein the storage member is provided with a storage member inner gas channel, the storage member inner gas channel is communicated with the reaction chamber, and the storage member inner gas channel is configured to be communicated with an external air pump. 
     
     
         20 . The microfluidic chip according to  claim 19 , wherein a first end of the storage member inner gas channel penetrates one end of the storage member provided with the groove, and the first end of the storage member inner gas channel is located between two adjacent storage chambers. 
     
     
         21 . The microfluidic chip according to  claim 8 , wherein the top cover is fixedly disposed at one end of the storage member provided with the groove, and the base is fixedly disposed at one end of the storage member away from the top cover. 
     
     
         22 . A microfluidic chip detection system, comprising a detection device and the microfluidic chip according to  claim 1 , wherein the detection device comprises an operating table for accommodating the microfluidic chip and an operating member for operating the valve.