US2025045806A1PendingUtilityA1

Activation of bioluminescence by structural complementation

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Assignee: PROMEGA CORPPriority: Mar 15, 2013Filed: Sep 23, 2024Published: Feb 6, 2025
Est. expiryMar 15, 2033(~6.6 yrs left)· nominal 20-yr term from priority
Inventors:Andrew S. DixonLance P. EncellMary HallKeith V. WoodMonika G. WoodMarie SchwinnBrock BinkowskiHicham ZegzoutiNidhi NathSubhanjan MondalSaid GoueliPoncho MeisenheimerThomas KirklandJames UnchDileep K. PulukkunatMatthew RobersMelanie DartThomas Machleidt
H04W 4/38H04W 4/35H04W 4/02G06Q 30/0631G06Q 30/0283A61K 51/08G01N 33/581G01N 33/542C12N 9/0069C07K 7/08C12Q 1/66C07K 14/43509C07K 2319/60G01N 33/533C07K 7/02C07K 19/00C07K 14/435
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Claims

Abstract

Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

Claims

exact text as granted — not AI-modified
1 . A detection reagent comprising:
 (a) a polypeptide comprising an amino acid sequence having less than 100% and greater than 40% sequence identity with SEQ ID NO: 440, wherein a detectable bioluminescent signal is produced in the presence of a substrate when the polypeptide contacts a peptide consisting of SEQ ID NO: 2 to form a bioluminescent complex, and   (b) the substrate for the bioluminescent complex.   
     
     
         2 . The detection reagent of  claim 1 , wherein the polypeptide comprises an amino acid sequence with at least 70% sequence identity to SEQ ID NO: 440. 
     
     
         3 . The detection reagent of  claim 1 , wherein the polypeptide comprises an amino acid sequence selected from the polypeptide sequences of Table 2. 
     
     
         4 . The detection reagent of  claim 2 , wherein the polypeptide comprises an amino acid sequence of SEQ ID NO: 1292. 
     
     
         5 . The detection reagent of  claim 1 , wherein the substrate is furimazine. 
     
     
         6 . A detection reagent comprising:
 (a) a peptide comprising an amino acid sequence having less than 100% and greater than 40% sequence identity with SEQ ID NO: 2, wherein a detectable bioluminescent signal is produced in the presence of a substrate when the peptide contacts a polypeptide consisting of SEQ ID NO: 440 to form a bioluminescent complex, and   (b) the substrate for the bioluminescent complex.   
     
     
         7 . The detection reagent of  claim 6 , wherein the peptide comprises an amino acid sequence with at least 70% sequence identity to SEQ ID NO: 2. 
     
     
         8 . The detection reagent of  claim 6 , wherein the peptide comprises an amino acid sequence is selected from the peptide sequences of Table 1. 
     
     
         9 . The detection reagent of  claim 8 , wherein the peptide comprises an amino acid sequence of SEQ ID NO: 390. 
     
     
         10 . The detection reagent of  claim 8 , wherein the peptide comprises an amino acid sequence of SEQ ID NO: 2271. 
     
     
         11 . A detection reagent comprising:
 (a) a polypeptide comprising an amino acid sequence of SEQ ID NO: 1292, and   (b) furimazine.

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