Limit size lipid nanoparticles and related methods

Abstract

Limit size lipid nanoparticles, methods for using the lipid nanoparticles, and methods and systems for making limit size lipid nanoparticles.

1 . A system, comprising:
 a first inlet microchannel configured to receive a first solution and provide a first stream comprising the first solution;   a second inlet microchannel configured to receive a second solution and provide a second stream comprising the second solution;   a third microchannel configured to receive the first stream and the second stream, wherein the third microchannel has a first region adapted for flowing the first stream and the second stream and a second region adapted for mixing the first stream and the second stream to provide a third stream comprising a mixture of the first solution and the second solution;   a first pump configured to provide the first solution to the first inlet microchannel at a first flow rate; and   a second pump configured to provide the second solution to the second inlet microchannel at a second flow rate,   wherein the ratio of the first flow rate to the second flow rate is 2.0 to 10.0 and wherein a combined flow rate of the first flow rate and second flow rate is from 2 ml/min to 1600 ml/min.