Bacteria-targeting capsid particle, treatment composition, disinfectant, food, bacteria elimination method, bactericidal method, corrosion prevention method, animal treatment method, gene introduction method, bacteria function addition method, method for producing bacteria-targeting capsid particle, and method for producing nucleic acid for bacteria-targeting capsid particle

Abstract

Provided is a bacteria-targeting capsid particle that is non-proliferative and has a high bactericidal effect. A capsid protein of a bacteriophage is prepared by a capsid nucleic acid element that synthesizes the capsid, which is divided from the bacteriophage genome and does not include a packaging region, but mainly includes the virion region. A bacteria-targeting capsid particle element (Bacteria-targeting capsid particle, B-CAP) is divided from a part of the bacteriophage genome other than the capsid nucleic acid element and includes a nucleic acid injection region, a replication region necessary for nucleic acid replication, and a packaging region. Although the assembled bacteria-targeting capsid particle (B-CAP) is non-proliferative, it can carry long DNA strands that give them new biological functions, such as bactericidal effects, or the other biological functions.

1 . A bacteria-targeting capsid particle comprising:
 a capsid protein of a bacteriophage; and   a bacteria-targeting capsid particle element including a nucleic acid injection region, a replication region necessary for replication of nucleic acid, and a packaging region in genome of the bacteriophage; and wherein being non-proliferative.   
     
     
         2 . The bacteria-targeting capsid particles according to  claim 1 , wherein
 the bacteria-targeting capsid particle element includes an exogenous gene.   
     
     
         3 . The bacteria-targeting capsid particles according to  claim 2 , wherein
 the exogenous gene includes one or any combination of a bactericidal gene, a biofilm-degrading gene, an antigen-presenting gene, and a gene for introduction.   
     
     
         4 . The bacteria-targeting capsid particles according to  claim 3 , wherein
 the bactericidal gene produces a secretory bactericidal product that sterilizes target bacteria and bacteria group around the target bacteria.   
     
     
         5 . The bacteria-targeting capsid particles according to  claim 4 , wherein
 the exogenous gene includes a resistance factor that suppresses the bactericidal effect on the target bacteria.   
     
     
         6 . A treatment composition comprising:
 the bacteria-targeting capsid particle according to  claim 1 .   
     
     
         7 . A disinfectant comprising:
 the bacteria-targeting capsid particle according to  claim 1 .   
     
     
         8 . A food comprising:
 the bacteria-targeting capsid particle according to  claim 1 .   
     
     
         9 . A bacteria elimination method comprising the step of:
 sterilizing target bacteria by the bacteria-targeting capsid particle according to  claim 1 .   
     
     
         10 . The bacteria elimination method according to  claim 9 , wherein the target bacteria are present in bacterial flora of a human, an animal, and/or an environment. 
     
     
         11 . The bacteria elimination method according to  claim 9 , wherein the target bacteria are present in food. 
     
     
         12 . A bactericidal method comprising the step of:
 sterilizing target bacteria with the bacteria-targeting capsid particle according to  claim 1 .   
     
     
         13 . A corrosion prevention method comprising the steps of:
 sterilizing target bacteria with the bacteria-targeting capsid particle according to  claim 1 ; and   preventing corrosion of an article.   
     
     
         14 . An animal treatment method comprising the step of:
 treating an animal with the bacteria-targeting capsid particle according to  claim 1 .   
     
     
         15 . A gene introduction method comprising the step of:
 introducing the exogenous gene included in the bacteria-targeting capsid particle according to  claim 2  into the target bacteria.   
     
     
         16 . A bacteria function addition method comprising the steps of:
 introducing the exogenous gene included in the bacteria-targeting capsid particle according to  claim 2  into the target bacteria; and   adding a function to the target bacteria.   
     
     
         17 . A method for producing bacteria-targeting capsid particle comprising the steps of:
 preparing a capsid protein of a bacteriophage by a capsid nucleic acid element that synthesizes a capsid without including a packaging region and is divided from genome of the bacteriophage;   packaging a bacteria-targeting capsid particle element, which includes a nucleic acid injection region, a replication region necessary for nucleic acid replication, and a packaging region and is divided from a part of the genome of the bacteriophage other than the capsid nucleic acid element, into the capsid protein.   
     
     
         18 . The method for producing the bacteria-targeting capsid particles according to  claim 17 , further comprising the steps of:
 introducing the capsid nucleic acid element and the bacteria-targeting capsid particle element into a preparation bacteria for preparation; and   producing the bacteria-targeting capsid particles in the preparation bacteria.   
     
     
         19 . (canceled) 
     
     
         20 . The method for producing the bacteria-targeting capsid particles according to  claim 18 , wherein
 the introducing the bacteria-targeting capsid particle element comprises introducing the bacteria-targeting capsid particle element by the chromosome or a plasmid.   
     
     
         21 . A method for producing nucleic acid for bacteria-targeting capsid particle comprising the steps of:
 preparing a capsid nucleic acid element that synthesizes a capsid without including a packaging region by dividing a genome of a bacteriophage;   preparing a bacteria-targeting capsid particle element including a nucleic acid injection region, a replication region necessary for replication of nucleic acid, and the packaging region by dividing a part of the genome of the bacteriophage other than the capsid nucleic acid element; and   constructing a nucleic acid for the bacteria-targeting capsid particle that is non-proliferative.   
     
     
         22 . The method for producing the bacteria-targeting capsid particles according to  claim 18 , wherein
 the introducing the capsid nucleic acid element comprises introducing the capsid nucleic acid element by a chromosome or an artificial chromosome.