Recombinant monoclonal antibodies and corresponding antigens for colon and pancreatic cancers

Abstract

The present invention provides for recombinant monoclonal antibodies that bind to human colorectal and pancreatic carcinoma-associated antigens, along with nucleic acid sequences encoding the antibody chains, and the amino acid sequences corresponding to the nucleic acids, and uses for these antibodies, nucleic acids and amino acids.

We claim: 
     
         1 - 24 . (canceled) 
     
     
         25 . An isolated cell comprising a nucleic acid molecule encoding a variable heavy chain polypeptide comprising the same CDRs of the variable heavy chain polypeptide encoded by the nucleic acid of SEQ ID NO:12 and a nucleic acid molecule encoding a variable light chain polypeptide comprising the same CDRs of the variable heavy chain polypeptide encoded by the sequence of SEQ ID NO:13, wherein said nucleic acid molecules provide for the expression of an antibody or antibody fragment which binds to the CPAA antigen. 
     
     
         26 . The isolated cell of  claim 25 , wherein said nucleic acid molecules are present in a vector. 
     
     
         27 . The isolated cell of  claim 25 , wherein said nucleic acid molecules are operably linked to regulatory regions which direct expression of said nucleic acid molecules. 
     
     
         28 . The isolated cell of  claim 25 , wherein said host cell is selected from bacterial, yeast, insect, fungal, avian and mammalian cells. 
     
     
         29 . The isolated cell of  claim 25 , which is a mammalian cell. 
     
     
         30 . The isolated cell of  claim 25 , which is selected from human, primate, hamster, rabbit, rodent, bovine, porcine, ovine, equine, caprine, canine and feline cells. 
     
     
         31 . The isolated cell of  claim 25 , which is a human cell. 
     
     
         32 . The isolated cell of  claim 25 , wherein said nucleic acid molecules respectively further encode heavy and light chain constant regions. 
     
     
         33 . The isolated cell of  claim 25 , wherein said nucleic acid molecules respectively further encode IgG1, IgG2, IgG3 or IgG4 heavy and light chain constant regions. 
     
     
         34 . The isolated cell of  claim 25 , wherein said nucleic acid molecules provide for the expression of a chimeric or humanized antibody or antibody fragment which binds to the CPAA antigen or a F(ab′) 2  or Fab which binds to the CPAA antigen. 
     
     
         35 . A method of producing an antibody or antibody fragment which binds to the CPAA antigen by culturing a cell according to  claim 25  under conditions that result in the expression of an antibody or antibody fragment encoded by said nucleic acid molecules. 
     
     
         36 . A method of producing an antibody or antibody fragment which binds to the CPAA antigen by culturing a cell according to  claim 26  under conditions that result in the expression of an antibody or antibody fragment encoded by said nucleic acid molecules. 
     
     
         37 . A method of producing an antibody or antibody fragment which binds to the CPAA antigen by culturing a cell according to  claim 27  under conditions that result in the expression of an antibody or antibody fragment encoded by said nucleic acid molecules. 
     
     
         38 . A method of producing an antibody or antibody fragment which binds to the CPAA antigen by culturing a cell according to  claim 28  under conditions that result in the expression of an antibody or antibody fragment encoded by said nucleic acid molecules. 
     
     
         39 . A method of producing an antibody or antibody fragment which binds to the CPAA antigen by culturing a cell according to  claim 29  under conditions that result in the expression of an antibody or antibody fragment encoded by said nucleic acid molecules. 
     
     
         40 . A method of producing an antibody or antibody fragment which binds to the CPAA antigen by culturing a cell according to  claim 30  under conditions that result in the expression of an antibody or antibody fragment encoded by said nucleic acid molecules. 
     
     
         41 . A method of producing an antibody or antibody fragment which binds to the CPAA antigen by culturing a cell according to  claim 31  under conditions that result in the expression of an antibody or antibody fragment encoded by said nucleic acid molecules. 
     
     
         42 . A method of producing an antibody or antibody fragment which binds to the CPAA antigen by culturing a cell according to  claim 32  under conditions that result in the expression of an antibody or antibody fragment encoded by said nucleic acid molecules. 
     
     
         43 . A method of producing an antibody or antibody fragment which binds to the CPAA antigen by culturing a cell according to  claim 33  under conditions that result in the expression of an antibody or antibody fragment encoded by said nucleic acid molecules. 
     
     
         44 . A method of producing an antibody or antibody fragment which binds to the CPAA antigen by culturing a cell according to  claim 34  under conditions that result in the expression of an antibody or antibody fragment encoded by said nucleic acid molecules.