Test composition for detecting urobilinogen

Abstract

4-Fluoro-3-nitrobenzenediazonium salts in test compositions capable of specific and sensitive determination of urobilinogen without any interference of bilirubin in an aqueous solution or body fluid such as urine. The diazonium salts have incorporated special stabilizing anions. In addition to a 4-fluoro-3-nitrobenzene diazonium salt, the test compositions preferably include an acid constituent capable of producing an acid pH upon contacting the solution to be tested. The test device comprises a bibulous carrier member, such as paper impregnated with the test composition in dry form.

What is claimed is: 
     
       1. Test composition for detecting urobilinogen in liquids, preferably in biological fluids, especially in urine, comprising a diazonium salt of the formula T ##SPC2## in which X -  represents a stabilizing anion selected from the group consisting of a chloride, sulfate, tetrafluoroborate, hexafluoroantimonate, hexafluoroantimony sulfonate, trifluoromethyl sulfonate, aryl sulfonate, or carboxylic acid radical and at least one solid acid and optionally a wetting agent and one or several stabilizers.   
     
     
       2. Test composition as claimed in claim 1, which contains additionally a wetting agent. 
     
     
       3. Test composition as claimed in claim 1, which contains additionally at least one stabilizer. 
     
     
       4. Test combination as claimed in claim 1, which contains additionally an optical brightener. 
     
     
       5. Test composition as claimed in claim 1, supported on an absorbent carrier. 
     
     
       6. Test composition as claimed in claim 1, which contains additionally a wetting agent and at least one stabilizer. 
     
     
       7. Test composition as claimed in claim 6, wherein the diazonium salt is 4-fluoro-3-nitrobenzene diazonium tetrafluoroborate, the acid is a mixture of metaphosphoric acid and citric acid, the wetting agent is dodecylbenzenesulfonic acid and the stabilizer is the disodium salt of naphthalene-1,5-disulfonic acid.