Hapten conjugated antibody for antibody or antigen detection

Abstract

The present invention encompasses an improved method for detecting antigen or antibody bound to a solid support which involves the reaction of hapten-labeled antibody to the antigen or antibody to be detected followed by reaction of the hapten moiety with labeled antibody to the hapten and the determination of the amount of label bound to the solid support.

What is claimed is: 
     
       1. In an immunoassay method for determining an antigen or an antibody from a test sample and bound to a solid support, the improvement comprising reacting the antigen or antibody bound to the solid support with an antibody having a hapten conjugated thereto and then reacting the hapten portion with labeled antihapten antibody, and measuring the labeled antihapten antibody bound to the solid support. 
     
     
       2. In an immunoassay method for determining hepatitis B surface antigen from a test sample and bound to a solid support, the improvement comprising reacting the hepatitis surface antigen bound to the solid support with an antibody to hepatitis B surface antigen, said antibody having a hapten conjugated thereto, reacting the hapten portion with labeled antihapten antibody, and measuring the labeled antihapten antibody bound to a solid support. 
     
     
       3. In an immunoassay method for determining hepatitis B antibody from a test sample and bound to a solid support, the improvement comprising reacting the hepatitis B antibody bound to the solid support with an antibody to the hepatitis B antibody, said antibody having a hapten conjugated thereto, reacting the hapten portion with labeled antihapten antibody, and measuring the labeled antihapten antibody bound to the solid support. 
     
     
       4. An immunoassay reagent which is antibody for hepatitis B surface antigen, said antibody having a hapten conjugated thereto. 
     
     
       5. An immunoassay reagent which is antibody to hepatitis B surface antigen, said antibody having a 2,4-dinitrophenyl moiety conjugated thereto.