Interferon inducer, a process for producing the same and pharmaceutical composition containing the same

Abstract

An interferon inducer isolated from the plant tissue, having an elemental analysis of H:7.4+/-0.4%, C: 45.6+/-0.4%, N: 13.5+/-0.4%, P: 2.8+/-0.3% and a molecular weight of from about 100,000 to about 3,000,000 (mainly about 200,000 to about 1,000,000) and containing as main chemical constituents amino acids and phosporic acid. This substance is believed to be a homogeneous polymer of protein and phosphoric acid. This substance has an excellent interferon inducing activity and low toxicity and is significantly active upon various viruses. This interferon inducer may be produced by extracting a substance having interferon inducing activity from the tissue of a plant of the genus Artemisia of the family Compositae or a variant thereof containing the said active substance with water and recovering the active substance from the extracted solution. The recovery may preferably be effected by ultrafiltration. Pharmaceutical composition of this invention comprises as active ingredient the said active substance in association with a pharmaceutical carrier or excipient.

We claim: 
     
       1. A process for producing a water-soluble interferon inducer from a plant tissue, comprising extracting said interferon inducer with water from the tissue of a plant belonging to the genus Artemisia containing said interferon inducer at a temperature of from ambient to the boiling point of the extraction mixture for a period of up to 5 days sufficient to extract the major portion of said interferon inducer present in said tissue, forming a supernatant from the extracted solution, fractionating the supernatant to yield fractions containing the major portion of said interferon inducer present in the supernatant, and recovering said interferon inducer therefrom. 
     
     
       2. The process of claim 1, wherein the plant is selected from Artemisia princeps Pamp; A. absinthium L.; A. maritima L.; a. kurramensis Qaz.; A. montana Pamp.; A. feddei Lev. et Van.; A. japonica Thun.; A. keisumeana Miq.; A. stolonifera Komar.; A. monophylla Kitam.; A. stelleriana Bess.; A. capillaris Thun.; A. vulgaris L.; A. abrotantum L.; A. vallesiaca All.; A. dracunculus L.; A. ludoviciana Nutt.; A. arbuscula Nutt.; A. tridentata Nutt.; A. filifolia Torr.; A. caudata Michx.; A. lindleyana Bess.; A. frigida Willd.; A. biennis Willd.; A. campestris L.; A. molinieri Quez and variants thereof.   
     
     
       3. The process of claim 1 wherein the extracting water has a pH of from 7 to 10. 
     
     
       4. The process of claim 3, wherein the pH is 7-10. 
     
     
       5. The process of claim 1, wherein the supernatant is fractionated by ultrafiltration. 
     
     
       6. The process of claim 5, wherein the ultrafiltration is effected by using an ultrafiltration membrane for retaining substances having a molecular weight of more than 100,000. 
     
     
       7. The process of claim 1, wherein the fractionation is effected by adding to the supernatant one member selected from hydrophilic organic solvents incapable of dissolving the said interferon inducer and ammonium salts so as to form a precipitate containing a major portion of the said interferon inducer. 
     
     
       8. The process of claim 7, wherein the organic solvent is selected from methanol, ethanol, propanol, butanol, acetone and mixture thereof at a concentration of 40-70 w/v %. 
     
     
       9. The process of claim 7, wherein the ammonium salt is selected from ammonium chloride, ammonium sulfate and cetylmetylammonium bromide at a concentration of 20-50 w/v %. 
     
     
       10. An amorphous whitish powder effective as an interferon inducer made by the process of claim 1. 
     
     
       11. A pharmacutical composition in dosage unit form, comprising as active ingredient an interferon inducer claimed in claim 10 in association with a pharmacutical carrier or excipient, said dosage unit containing from about 0.001 mg to 13 g of the said active ingredient. 
     
     
       12. The interferon inducer of claim 10 having a molecular weight of about 100,000 to about 3,000,000. 
     
     
       13. A pharmaceutical composition comprising as an active ingredient an interferon inducer made by the process of claim 1 in association with a pharmaceutical carrier or excipient. 
     
     
       14. The pharmaceutical composition of claim 13 wherein said interferon inducer has a molecular weight of about 100,000 to about 3,000,000. 
     
     
       15. The process of claim 1 wherein said interferon inducer has a molecular weight of about 100,000 to about 3,000,000.