Antibiotics produced by fermentation of a novel strain of Streptomyces hygroscopicus

Abstract

Antibiotic Bu-2659 complex, containing components A, B, C, D and E, is produced by cultivation of Streptomyces hygroscopicus Strain No. J296-21, ATCC No. 39150.

We claim: 
     
       1. A process for the preparation of antibiotic Bu-2659 complex which comprises cultivating a strain of Streptomyces hygroscopicus ATCC No. 39150 under submerged aerobic conditions in an aqueous nutrient medium containing assimilable sources of carbon and nitrogen at a temperature of from about 20° C. to about 43° C. until a substantial amount of Bu-2659 complex is produced and accumulated in the culture medium, and recovering the Bu-2659 complex from the culture medium. 
     
     
       2. The process of claim 1 which includes the additional step of separating Bu-2659 complex into components A, B, C, D and E by chromatographic means. 
     
     
       3. The process of claim 3 wherein the cultivation is conducted at a temperature of from about 28° C. to about 37° C. 
     
     
       4. A biologically pure culture of the Bu-2659 complex-producing microorganism Streptomyces hygroscopicus Strain No. J296-21, ATCC No. 39150.