Process for preparation of substance having hypotensive component

Abstract

Disclosed is a process for the preparation of a substance having a hypotensive component, which comprises mixing and extracting an oil-squeezed component from a soybean oil-preparing step with water, an aqueous solution of an acid, an aqueous solution of a salt or an aqueous solution of an acid and a salt, subjecting the extract to centrifugal separation, separating a hypotensive component from the aqueous phase, and, if necessary, drying the separated hypotensive component. This substance is valuable as a medicine or in the field of health-promoting foods.

I claim: 
     
       1. A process for the preparation of a substance having a hypotensive component, which comprises (i) mixing an oil component squeezed or extracted from a soybean, with an aqueous extraction medium composed of (A) water, (B) an aqueous solution containing a salt selected from the group consisting of ammonium sulfate and sodium chloride in the concentration of 0.1 to 3%, (C) an aqueous solution containing an acid selected from the group consisting of lactic acid, acetic acid, succinic acid, oxalic acid, malic acid and citric acid in the concentration of up to 10% or (D) a combined aqueous solution of (B) and (C), said extraction medium being used in an amount of at least 5% based on the oil component, at such a temperature that the temperature of the mixture is lower than 90° C. at the initial stage but is lowered below 50° C. at the final stage, thereby to extract said substance in the aqueous extraction medium, (ii) subjecting the mixture to centrifugal separation to separate the aqueous extract from the oil phase, and (iii) recovering the substance in the solid state from the aqueous extract, said steps (i) and (ii) being carried out under temperature-time conditions included within the operation region A shown in FIG. 4. 
     
     
       2. A process for the preparation of a substance having a hypotensive component according to claim 1, wherein the oil component is a miscella, a solvent-removed oil (95%), a crude oil, a degummed oil or a gum. 
     
     
       3. A process for the preparation of the substance having a hypotensive component according to claim 2 wherein the oil component is a miscella. 
     
     
       4. A process for the preparation of the substance having a hypotensive component according to claim 2 wherein the oil component is a solvent-removed oil (95%). 
     
     
       5. A process for the preparation of the substance having a hypotensive component according to claim 2 wherein the oil component is a crude oil. 
     
     
       6. A process for the preparation of the substance having a hypotensive component according to claim 2 wherein the oil component is a degummed oil. 
     
     
       7. A process for the preparation of the substance having a hypotensive component according to claim 2 wherein the oil component is a gum. 
     
     
       8. A process for the preparation of a substance having a hypotensive component according to claim 1 wherein the step of recovering the substance having a hypotensive component in the solid state from the aqueous extract comprises concentrating the aqueous extract under temperature-time conditions such that the aqueous extract is not exposed to a temperature higher than 80° C. for any longer than 30 minutes. 
     
     
       9. A process for the preparation of a substance having a hypotensive component according to claim 8 wherein the concentration of the aqueous extract is carried out continuously. 
     
     
       10. A process for the preparation of a substance having a hypotensive component according to claim 1, wherein the separation of the hypotensive component is accomplished by salting-out. 
     
     
       11. A process for the preparation of a substance having a hypotensive component according to claim 10, wherein the salting-out is performed by using ammonium sulfate, sodium chloride or a mixture thereof. 
     
     
       12. A process for the preparation of a substance having a hypotensive component according to claim 1, wherein the separation of the hypotensive component is accomplished by concentration and salting-out in combination. 
     
     
       13. A process for the preparation of a substance having a hypotensive component according to claim 1, which further comprises drying the substance obtained in step (iii) under a reduced pressure under temperature-time conditions included with the operation region A shown in FIG. 4. 
     
     
       14. The process of claim 1 wherein the aqueous extraction medium is composed of (A) water. 
     
     
       15. The process of claim 1 wherein the aqueous extraction medium is composed of (B) the aqueous solution containing ammonium sulfate or sodium chloride. 
     
     
       16. The process of claim 1 wherein the aqueous extraction medium is composed of (C) the aqueous solution containing lactic acid, acetic acid, succinic acid, oxalic acid, malic acid or citric acid. 
     
     
       17. The process of claim 1 wherein the aqueous extraction medium is composed (D) the combined aqueous solution of (B) and (C).