Wound-healing composition

Abstract

A soft tissue wound healing composition comprising an aqueous mixture of fibrillar collagen, heparin, and undegranulated platelets or platelet releasate. The composition is applied topically to the wound site in conjunction with means to keep it at the site and hydrated or in the form of an occlusive dressing.

We claim: 
     
       1. A soft tissue wound healing composition comprising an aqueous mixture of: (a) fibrillar collagen at a concentration in the range of about 1 to about 70 mg/ml;   (b) about 0.1% to about 10% by weight based on collagen of a glycosaminoglycan selected from the group consisting of heparin, a heparin-like glycosaminoglycan, and mixtures thereof; and   (c) about 0.01% to about 10% by volume based on collagen of undegranulated platelets or an equivalent amount of platelet releasate.   
     
     
       2. A composition for promoting fibroplasia at a soft tissue wound site comprising an aqueous mixture of: (a) fibrillar collagen at a concentration in the range of about 1 to about 70 mg/ml;   (b) about 0.1% to about 10% by weight based on collagen of a glycosaminoglycan selected from the group consisting of heparin, a heparin-like glycosaminoglycan, and mixtures thereof; and   (c) about 0.01% to about 10% by volume based on collagen of undegranulated platelets or an equivalent amount of platelet releasate.   
     
     
       3. A composition for promoting reepithelialization at a soft tissue wound site comprising an aqueous mixture of: (a) fibrillar collagen at a concentration in the range of about 1 to about 70 mg/ml;   (b) about 0.1% to about 10% by weight based on collagen of a glycosaminoglycan selected from the group consisting of heparin, a heparin-like glycosaminoglycan, and mixtures thereof; and   (c) about 0.01% to about 10% by volume based on collagen of undegranulated platelets or an equivalent amount of platelet releasate.   
     
     
       4. A composition for promoting vascularization at a soft tissue wound site comprising an aqueous mixture of: (a) fibrillar collagen at a concentration in the range of about 1 to about 70 mg/ml;   (b) about 0.1% to about 10% by weight based on collagen of a glycosaminoglycan selected from the group consisting of heparin, a heparin-like glycosaminoglycan, and mixtures thereof; and   (c) about 0.01% to about 10% by volume based on collagen of undegranulated platelets or an equivalent amount of platelet releasate.   
     
     
       5. A cutaneous wound healing composition comprising an aqueous mixture of: (a) fibrillar collagen at a concentration in the range of about 1 to about 70 mg/ml;   (b) about 0.1% to about 10% by weight based on collagen of heparin; and   (c) about 0.01% to about 10% by volume based on collagen of undegranulated platelets or an equivalent amount of platelet releasate.   
     
     
       6. The composition of claim 5 wherein the collagen is Type I collagen, Type III collagen, or mixtures thereof. 
     
     
       7. The composition of claim 6 wherein the fibrillar collagen is reconstituted fibrillar collagen. 
     
     
       8. The composition of claim 5 wherein the concentration of fibrillar collagen is in the range of 15 to 35 mg/ml. 
     
     
       9. The composition of claim 5 wherein the amount of heparin in the composition is in the range of 0.3% to 3% by weight based on collagen and the amount of undegranulated platelets is 2% to 10% by volume based on collagen and the amount of platelet releasate is equivalent to said amount of undegranulated platelets. 
     
     
       10. The composition of claim 5 wherein the collagen is reconstituted xenogeneic collagen, the concentration of collagen is in the range of 15 to 35 mg/ml, the heparin constitutes about 1% by weight based on collagen, and the amount of undegranulated platelets is about 2% to 10% by volume based on collagen and the amount of platelet releasate is equivalent to said amount of undegranulated platelets. 
     
     
       11. A cutaneous wound dressing comprising a composite of (a) a solid support on which is carried   (b) the composition of claim 5.   
     
     
       12. A cutaneous wound dressing comprising a composite of (a) a solid support on which is carried   (b) the composition of claim 6.   
     
     
       13. A cutaneous wound dressing comprising a composite of (a) a solid support on which is carried   (b) the composition of claim 7.   
     
     
       14. A cutaneous wound dressing comprising a composite of (a) a solid support on which is carried   (b) the composition of claim 8.   
     
     
       15. A cutaneous wound dressing comprising a composite of (a) a solid support on which is carried   (b) the composition of claim 9.   
     
     
       16. A cutaneous wound dressing comprising a composite of (a) a solid support on which is carried   (b) the composition of claim 10.   
     
     
       17. A method of promoting healing of a soft tissue wound comprising applying the composition of claim 1 to the wound site in conjunction with means for keeping the composition at the site and hydrated. 
     
     
       18. The method of claim 17 wherein the soft tissue wound is a cutaneous wound, a dermal wound, a mucosal wound, or an epithelial wound. 
     
     
       19. A method of promoting healing of a cutaneous wound comprising applying the composition of claim 5 to the wound site in conjunction with a dressing that keeps the composition at the site and hydrated. 
     
     
       20. A method of promoting healing of a cutaneous wound comprising applying the composition of claim 6 to the wound site in conjunction with a dressing that keeps the composition at the site and hydrated. 
     
     
       21. A method of promoting healing of a cutaneous wound comprising applying the composition of claim 7 to the wound site in conjunction with a dressing that keeps the composition at the site and hydrated. 
     
     
       22. A method of promoting healing of a cutaneous wound comprising applying the composition of claim 8 to the wound site in conjunction with a dressing that keeps the composition at the site and hydrated. 
     
     
       23. A method of promoting healing of a cutaneous wound comprising applying the composition of claim 9 to the wound site in conjunction with a dressing that keeps the composition at the site and hydrated. 
     
     
       24. A method of promoting healing of a cutaneous wound comprising applying the composition of claim 10 to the wound site in conjunction with a dressing that keeps the composition at the site and hydrated.