Monoclonal antibody specific for a mammary tumor cell surface antigen

Abstract

Hybridomas are provided which secrete an IgG1 or IgG2 monoclonal antibody which binds to an epitope on an antigen, which occurs in the plasma membrane of MCF-7 cells and has a molecular weight of 22 kD. The epitope of the antibodies is exposed on the extracellular side of the plasma membrane of the MCF-7 cells. The monoclonal antibodies, which react generally with human mammary carcinoma cells, but with few non-mammary cancer cells, are useful diagnostically and therapeutically.

What is claimed is: 
     
       1. A culture of an hybridoma which is a culture with the characteristics of the culture of hybridoma 15A8, on deposit at the ATCC under deposit number HB-8655, or an antibody-producing subculture of such culture, wherein said hybridoma secretes an IgG1 or IgG2 antibody which binds to an epitope on an antigen which occurs in the plasma membrane of MCF-7 cells, said epitope being exposed on the extracellular side of said membrane on said cells in culture, and said antigen having a molecular weight of approximately 22 kD; wherein the binding of the secreted antibody to said epitope is substantially eliminated by treatment of said antigen with an aldehyde fixative or denaturation of said antigen with heat. 
     
     
       2. A culture of hybridoma which is a culture with the characteristics of the culture of hybridoma 15A8G 2a , on deposit at the ATCC under deposit number HB-9344or an antibody-producing subculture of such culture, wherein said hybridoma secretes an IgG1 or IgG2 antibody which binds to an epitope on an antigen which occurs in the plasma membrane of MCF-7 cells, said epitope being exposed on the extracellular side of said membrane on said cells in culture, and said antigen having a molecular weight of approximately 22 kD; wherein the binding of the secreted antibody to said epitope is substantially eliminated by treatment of said antigen with an aldehyde fixative or denaturation of said antigen with heat. 
     
     
       3. A culture of hybridoma which is a culture with the characteristics of the culture of hybridoma 15A8G 2b , on deposit at the ATCC under deposit number HB-9345or an antibody-producing subculture of such culture; wherein said hybridoma secretes an IgG1 or IgG2 antibody which binds to an epitope on an antigen which occurs in the plasma membrane of MCF-7 cells, said epitope being exposed on the extracellular side of said membrane on said cells in culture, and said antigen having a molecular weight of approximately 22 kD; wherein the binding of the secreted antibody to said epitope is substantially eliminated by treatment of said antigen with an aldehyde fixative or denaturation of said antigen with heat. 
     
     
       4. A monoclonal IgG1 or IgG2 antibody produced by a culture with the characteristics of the culture of hybridoma 15A8, on deposit at the ATCC under deposit number HB-8655, or an antibody-producing subculture of such culture, wherein said antibody binds to an epitope on an antigen which occurs in the plasma membrane of MCF-7 cells, said epitope being exposed on the extracellular side of said membrane on said cells in culture, and said antigen having a molecular weight of approximately 22 kD, wherein the binding of the secreted antibody to said epitope is substantially eliminated by treatment of said antigen with an aldehyde fixative or denaturation of said antigen with heat. 
     
     
       5. A monoclonal IgG1 or IgG2 antibody produced by a culture with the characteristics of the culture of hybridoma 15A8G 2a , on deposit at the ATCC under deposit number HB-9344, or an antibody-producing subculture of such culture, wherein said antibody binds to an epitope on an antigen which occurs in the plasma membrane of MCF-7 cells, said epitope being exposed on the extracellular side of said membrane on said cells in culture, and said antigen having a molecular weight of approximately 22 kD, wherein the binding of the secreted antibody to said epitope is substantially eliminated by treatment of said antigen with an aldehyde fixative or denaturation of said antigen with heat. 
     
     
       6. A monoclonal IgG1 or IgG2 antibody produced by a culture with the characteristics of the culture of hybridoma 15A8G 2b , on deposit at the ATCC under deposit number HB-9345, or an antibody-producing subculture of such culture, wherein said antibody binds to an epitope on an antigen which occurs in the plasma membrane of MCF-7 cells, said epitope being exposed on the extracellular side of said membrane on said cells in culture, and said antigen having a molecular weight of approximately 22 kD, wherein the binding of the secreted antibody to said epitope is substantially eliminated by treatment of said antigen with an aldehyde fixative or denaturation of said antigen with heat. 
     
     
       7. An antibody according to claim 4 which is of isotype IgG1. 
     
     
       8. An antibody according to claim 5 which is of isotype IgG 2a . 
     
     
       9. An antibody according to claim 6 which is of isotype IgG 2b .