Serine proteinase inhibitor gene from the insect Manduca sexta

Abstract

Disclosed is a cDNA clone isolated from a fat body cDNA library from the tobacco hornworm Manduca sexta, and the deduced amino acid sequence of the serine proteinase inhibitor encoded by the cDNA.

What is claimed is: 
     
       1. A purified DNA molecule having a sequence defined by nucleotides 25-1200 of FIG. 3 which encodes for Manduca sexta hemolymph serine proteinase inhibitor. 
     
     
       2. The purified DNA sequence of claim 1 further including a 5'-untranslated eukaryotic initiation site and a 3'-untranslated eukaryotic polyadenylation signal, said eukaryotic initiation site consisting essentially of the nucleotide sequence CGAAC appearing at nucleotide positions 20-24 of FIG. 3 and said polyadenylation signal consisting essentially of the nucleotide sequence AATAAA appearing at nucleotide positions 1414-1419 of FIG. 3. 
     
     
       3. A purified DNA sequence complementary to the sequence of claim 1. 
     
     
       4. An isolated and purified DNA molecule encoding the amino acid sequence for Manduca sexta hemolymph serine proteinase inhibitor as set forth in FIG. 3.