Method of plant tissue culture

Abstract

PCT No. PCT/JP89/00741 Sec. 371 Date Mar. 21, 1990 Sec. 102(e) Date Mar. 21, 1990 PCT Filed Jul. 25, 1989 PCT Pub. No. WO90/00855 PCT Pub. Date Feb. 8, 1990.A method of plant tissue culture which comprises culturing a tissue or an organ of a plant, a part of the same or cultured cells in a medium containing a culture filtrate and/or an extract of a photosynthetic procaryotic microorganism. Such a culturing method effectively proliferates the plant tissues and cultured cells, and also promotes the formation of adventive embryos, regeneration of the plant body and production of useful substances formed by that plant. Strains of cyanobacteria or photosynthetic bacteria are preferably used as the photosynthetic procaryotic microorganism.

We claim: 
     
       1. A method of culturing plant tissue, which comprises culturing at least part of a plant tissue, at least part of a plant organ or cultured cells thereof in a culture medium containing one or more members selected from the group consisting of a culture filtrate from a photosynthetic procaryotic microorganism, an extract from said microorganism, a basic substance isolated from said culture filtrate, a basic substance isolated from said extract, a high molecular weight fraction from said culture filtrate, a high molecular weight fraction from said extract, a low molecular weight fraction from said culture filtrate and a low molecular weight fraction from said extract, wherein said culture filtrate is obtained by filtration or centrifugation of a culture solution obtained by cultivation of said microorganism, said extract is obtained by bringing microbial cells of said microorganism or disrupted microbial cells thereof into contact with a solvent, and each of said basic substances is isolated from said filtrate or extract through fractionation by use of an organic solvent. 
     
     
       2. The method as defined in claim 1, wherein said culturing is conducted to thereby proliferate said plant tissue, plant organ or cultured cells thereof. 
     
     
       3. The method as defined in claim 1, wherein said culturing is conducted to thereby form adventive embryos of said plant tissue, plant organ or cultured cells thereof. 
     
     
       4. The method as defined in claim 1, wherein said culturing is conducted to thereby regenerate a plant body. 
     
     
       5. The method as defined in claim 4, wherein calluses are cultured, thereby regenerating said plant body. 
     
     
       6. The method as defined in claim 4, wherein adventive embryos are cultured, thereby regenerating said plant body. 
     
     
       7. The method as defined in claim 4, wherein tissue pieces are cultured, thereby regenerating said plant body. 
     
     
       8. The method as defined in claim 4, wherein protocorm-like bodies are cultured, thereby regenerating said plant body. 
     
     
       9. The method as defined in claim 1, wherein said culturing is conducted to thereby produce a natural organic compound formed by said plant tissue, plant organ or cultured cells thereof. 
     
     
       10. The method as defined in claim 1, wherein said culture medium contains one or both of said basic substances. 
     
     
       11. The method as defined in claim 1, wherein said culture medium contains one or more of said high and low molecular weight fractions, said fractions being obtained by subjecting said culture filtrate or extract to dialysis, gel filtration or ultrafiltration, and fractionating the resulting product on the basis of molecular weight to obtain said high molecular weight fraction or said low molecular weight fraction. 
     
     
       12. The method as defined in claim 1, wherein said photosynthetic procaryotic microorganism is a strain of cyanobacteria. 
     
     
       13. The method as defined in claim 12, wherein said cyanobacteria is Synechococcus. 
     
     
       14. The method as defined in claim 1, wherein said photosynthetic procaryotic microorganism is a strain of photosynthetic bacteria. 
     
     
       15. The method as defined in claim 14, wherein said photosynthetic bacteria is Rhodopseudomonas.