Composition for measurement of potassium ion concentration and composition for elimination of ammonium ions

Abstract

There is disclosed a composition for measurement of potassium ion concentration, which contains a) pyruvate kinase, b) glycerol kinase, c) glycerol-3-phosphate oxidase, d) peroxidase, e) a chromogen of reduced type, f) adenosine diphosphate or a salt thereof, and g) phosphoenolpyruvic acid or a salt thereof. Also disclosed is a composition for elimination of ammonium ions, which contains a) glutamate dehydrogenase, b) glucose dehydrogenase, and c) nicotinamide adenine dinucleotide of reduced type (NADH) or nicotinamide adenine dinucleotide phosphate of reduced type (NADPH).

What is claimed is: 
     
       1. A composition for measurement of potassium ion concentration, which comprises a) pyruvate kinase, b) glycerol kinase, c) glycerol-3-phosphate oxidase, d) peroxidase, e) a chromogen of reduced type, e) adenosine diphosphate or a salt thereof, and e) phosphoenolpyruvic acid or a salt thereof, said composition being suitable for the measurement of potassium ion concentration. 
     
     
       2. A composition according to claim 1, which comprise a) pyruvate kinase at a concentration of from 0.01 to 10 μ/ml, b) glycerol kinase at a concentration of from 0.05 to 50 μ/ml, c) glycerol-3-phosphate oxidase at a concentration of from 0.1 to 50 μ/ml, d) peroxidase at a concentration of from 1 to 100 μ/ml, e) a chromogen of reduced type at a concentration of from 0.01 to 10 mM, f) adenosine diphosphate or a salt thereof at a concentration of from 1 to 20 mM, and g) phosphoenolpyruvic acid or a salt thereof at a concentration of from 0.5 to 5 mM. 
     
     
       3. A composition for elimination of ammonium ions, which comprises a) glutamate dehydrogenase, b) glucose dehydrogenase, and c) nicotinamide adenine dinucleotide of reduced type (NADH) or nicotinamide adenine dinucleotide phosphate of reduced type (NADPH), said composition being suitable for the elimination of ammonium ions. 
     
     
       4. A composition according to claim 3, which comprises a) glutamate dehydrogenase at a concentration of from 20 to 200 μ/ml, b) glucose dehydrogenase at a concentration of from 10 to 100 μ/ml, and c) nicotinamide adenine dinucleotide of reduced type (NADH) of nicotinamide adenine dinucleotide phosphate of reduced type (NADPH) at a concentration of from 0.005 to 0.5 mM. 
     
     
       5. A combination of a first composition for measurement of potassium ion concentration and a second composition for elimination of ammonium ions, the first composition comprising a) pyruvate kinase, b) glycerol kinase, c) glycerol-3-phosphate oxidase, d) peroxidase, e) a chromogen of reduced type, e) adenosine diphosphate or a salt thereof, and e) phosphoenolpyruvic acid or a salt thereof, and the second composition comprising a) glutamate dehydrogenase, b) glucose dehydrogenase, and c) nicotinamide adenine dinucleotide of reduced type (NADH) or nicotinamide adenine dinucleotide phosphate of reduced type (NADPH), said combination being suitable for the measurement of potassium ion concentration and for the elimination of ammonium ions. 
     
     
       6. A method for the measurement of potassium ion concentration in a body fluid sample, said method comprising reacting said body fluid sample with a composition comprising a) pyruvate kinase, b) glycerol kinase, c) glycerol-3-phosphate oxidase, d) peroxidase, e) a chromogen of reduced type, f) adenosine diphosphate or a salt thereof, and g) phosphoenolpyruvic acid or a salt thereof and thereafter performing a colorimetric analysis. 
     
     
       7. The method according to claim 6, wherein said body fluid sample is a blood serum sample. 
     
     
       8. The method according to claim 6, wherein said pyruvate kinase is in a concentration in said composition from about 0.01 to about 10 μ/ml, said glycerol kinase is in a concentration in said composition from about 0.05 to about 50 μ/ml, said glycerol-3-phosphate oxidase is in a concentration in said composition from about 0.1 to about 50 μ/ml, said peroxidase is in a concentration in said composition from about 1 to about 100 μ/ml, said chromogen is in a concentration in said composition from about 0.1 to about 10 mM, said adenosine diphosphate or salt thereof is in a concentration in said composition from about 1 to about 20 mM, and said phosphoenolpyruvic acid or salt thereof is in a concentration in said composition from about 0.5 to about 5 mM. 
     
     
       9. A method for the elimination of ammonium ions in a body fluid sample, said method comprising reacting said body fluid sample with a composition comprising) glutamate dehydrogenase, b) glucose dehydrogenase, and c) nicotinamide adenine dinucleotide of reduced type (NADH) or nicotinamide adenine dinucleotide phosphate of reduced type (NADPH). 
     
     
       10. The method according to claim 9, wherein said body fluid sample is a blood serum sample. 
     
     
       11. The method according to claim 9, wherein said glutamate dehydrogenase concentration in said composition from about 20 to about 200 μ/ml, said glucose dehydrogenase concentration in said composition from about 10 to about 100 μ/ml, and said NADH or said NADPH concentration in said composition from about 0.005 to about 0.5 mM. 
     
     
       12. A method for the simultaneous elimination of ammonium ions in a body fluid sample and measurement of potassium ion concentration in said body fluid sample, said method comprising reacting said body fluid sample with a composition comprising a) pyruvate kinase, b) glycerol kinase, c) glycerol-3-phosphate oxidase, d) peroxidase, e) a chromogen of reduced type, f) adenosine diphosphate or a salt thereof, g) phosphoenolpyruvic acid or a salt thereof, h) glutamate dehydrogenase, i) glucose dehydrogenase, and j) nicotinamide adenine dinucleotide of reduced type (NADH) or nicotinamide adenine dinucleotide phosphate of reduced type (NADPH) and thereafter performing a colorimetric analysis. 
     
     
       13. The method according to claim 12, wherein said body fluid sample is a blood serum sample. 
     
     
       14. The method according to claim 6, wherein said composition further comprises phenol. 
     
     
       15. The method according to claim 6 wherein said composition further comprises a buffer to maintain a pH of said composition of from about 6 to about 9. 
     
     
       16. The method according to claim 15, wherein said buffer is selected from the group consisting of triethanolamine buffer, Good's buffer and tris buffer. 
     
     
       17. The method according to claim 6, wherein said composition further comprises a preservative. 
     
     
       18. The method according to claim 17, wherein said preservative is selected from the group consisting of NaN 3  and antibiotics. 
     
     
       19. The method according to claim 6, wherein said composition further comprises a surfactant. 
     
     
       20. The method according to claim 19, wherein said surfactant is a nonionic surfactant. 
     
     
       21. The method according to claim 6, wherein said composition further comprises a stabilizer and enzyme activator. 
     
     
       22. The method according to claim 21, wherein said stabilizer and enzyme activator is selected from the group consisting of albumin, flavin adenine dinucleotide (FAD) and magnesium ions. 
     
     
       23. The method according to claim 6, wherein said reaction is carried out at from about 10° to about 40° C.