Monoclonal antibody specific for human interleukin-1β and method for detection of biologically active human interleukin-1β

Abstract

The present invention provides a monoclonal antibody characterized in that the antibody has specific reactivity on human IL-1β, and a process for producing the antibody. The invention also provides a method for detection of biologically active human IL-1β.

We claim: 
     
       1. Monoclonal antibody 1β GOM43-4 produced by hybridoma cell line FERM BP-2565. 
     
     
       2. A hybridoma cell line, FERM BP-2565, that produces the monoclonal antibody of claim 1. 
     
     
       3. An immunoassay method for detecting biologically active human interleukin-1β which comprises the steps of: (a) contacting a sample suspected of containing human interleukin-1β with a monoclonal antibody as defined in claim 1 in order to form an immune complex; and   (b) determining the presence of said immune complex.