US5366895AExpiredUtility

Method for lysing liposomes using polyethyleneglycol monononylphenyl ethers

42
Assignee: BECTON DICKINSON COPriority: Mar 5, 1990Filed: Mar 5, 1990Granted: Nov 22, 1994
Est. expiryMar 5, 2010(expired)· nominal 20-yr term from priority
Inventors:Herman RutnerAbdul M. ButtJosephine D. ReadioLewis Pollack
G01N 33/586Y10S436/80Y10S436/829Y10T436/10Y10T436/107497
42
PatentIndex Score
12
Cited by
9
References
11
Claims

Abstract

A method for rapidly lysing liposomes having transition temperatures in the range of 35° to 65° C. is provided. Such liposomes are treated with a surfactant including ##STR1## wherein x represents an average of 9 or 12. The method is applicable to fluorescence immunoassay procedures.

Claims

exact text as granted — not AI-modified
What is claimed is: 
     
       1. A method for lysing liposomes comprising saturated fatty acid phosphatides and having transition temperatures in the range of 35° C. to 65° C. comprising treating the liposomes to be lysed with a surfactant selected from ##STR6## wherein the average value of x is 9 or 12. 
     
     
       2. A method according to claim 1 wherein the average value of x is 9. 
     
     
       3. A method according to claim 1 wherein the average value of x is 12. 
     
     
       4. A method according to claim 1 wherein said liposomes contain C 18  :0 fatty acid phosphatides as one of the membrane constituents of said liposomes. 
     
     
       5. A method according to claim 1 wherein said liposomes contain C 18  :0 distearoyl phosphatides as one of the membrane constituents of said liposomes. 
     
     
       6. A method according to claim 1 including the step of binding said liposomes to a surface prior to treating said liposomes with said surfactant. 
     
     
       7. A method according to claim 6 wherein said liposomes contain a fluorescent dye. 
     
     
       8. A method according to claim 1 wherein said liposomes contain a fluorescent dye, including the step of measuring the fluorescence released from said liposomes subsequent to treating said liposomes with said surfactant. 
     
     
       9. A method according to claim 2 wherein said liposomes contain a fluorescent dye, including the step of measuring the fluorescence released from said liposomes subsequent to treating said liposomes with said surfactant. 
     
     
       10. A method according to claim 3 wherein said liposomes contain a fluorescent dye, including the step of measuring the fluorescence released from said liposomes subsequent to treating said liposomes with said surfactant. 
     
     
       11. A method according to claim 1 wherein said liposomes are digoxin liposomes.

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