US5382527AExpiredUtility
Transposable elements from streptomyces and other organisms
Est. expirySep 30, 2008(expired)· nominal 20-yr term from priority
Inventors:Patricia J. Solenberg
C12N 15/76C12N 15/00
29
PatentIndex Score
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Cited by
29
References
11
Claims
Abstract
A novel method for isolating transposable elements was used to isolate an approximately 1.6 kb insertion sequence from Streptomyces. The method entails transforming a cell with a plasmid containing a repressor gene, so that the introduction of a transposable element into the gene allows the expression of a selectable marker in a second host cell. The novel insertion sequence isolated from Streptomyces lividans CT2 has been designated IS493.
Claims
exact text as granted — not AI-modifiedI claim:
1. A recombinant DNA compound comprising the transposable element IS493, wherein one strand of the DNA sequence comprises: ______________________________________
5'-AGCGTTTTCC
ATCCTCAAGC TGAGCTGGCC
TGATGCAGGC AGAGGACGGC CTGGACGAGG
ACCGTGACGC GGGTGGTCGA GCAACGGAGC
CTGCGAAGGA GTCGCCAGGA CTTGAGGGTC
GCGACGGCTT GCTCGACGAG CGCCCGGATC
TTCGCGTGGG ATCGATTGAC GGCCTGCTGC
CCTGCAGAGA GCGTCTCCCA CCGGCCGCGG
TAGGGAACAC GGACCGTGCC TCCGGCGCCC
TGGTAGCCCT TGTCTGCCCA GCAGTTGACG
TCGGCTGTGG CAAGCGTGTC GATGATGCCG
TGCTCGCGGG CCGCACGGAC GTCGTGGACG
GCTCCGGGCA GGGCCGGCGA GGCCCACAGC
AGGCGGCCGG AAGGATCTGC GATCACCTGT
ACGTTCATCC CGTGTTTCTT GTGCTTGCCC
GAGTAGAAAG GCCGGTCGGC GGCGATTCGG
TCGATCGGCA GCAGCGTCCC GTCCAGCAGG
ACGAACGCCT TCATCGACGC CGCCCGGACC
GCCTCGGCGA GCGTCGGGGC AAGGGCGGCC
AGAACCTCAG CTGCCTCGGT GACGTAGCGG
TAGGCAGTGG TGGTGCCCAC GCCGAACCCG
GCCGCGAGCT GCACATACGG ATGACCGTTG
CGGAGATGGG CGAGGGCGAG CAGAGCCTGC
CGACCTGCGC TCAGGCGCCG CCACCGGGTG
CCGAGACCAC GGCGGTGTTC CCGCAGACGG
GCGGAGAGGA AGCGGAGGGC AGAACTGGAC
ACGTCCAGCC CGGAGGGGTA AACAAGCACG
TGAAGCCTCT AGTAGAGACG GATTTCTTGG
TCGAAAACCC ATCTACCAGG GGCTTCACCC
CTGTGTCAGG CCAACCCGCA AGCTGCCGAA
GCAGAATGAA GACGCTCCTT GGCTACCGGT
CTGGCGCATG CGAACCCCGA CGTCGTGGGT
CTAGCTGGTT GTCCTGACGG GTGCGCTGCT
GCGCCCGTGG CGTCGACCGC TGAGCTCGGC
GTCGAGTGTC TCCTGAGCCA CGCGCATGGC
CTCGGCGTAT ACGGGGTCCT GCAGCCGCTT
CCACATCTCG CCCTCAGAGA CGICCTCAAC
GCAGCTGACC ACCCACCAGA TATTGCCGAA
GGGGTCCTTG ATGCGCCCTC CGCGCTGTCC
GAAGGCGTCG TCGGCCAGGG AAGTGACGAC
ATAGCCGCCA GCTGCGACGG CCTGGGAAAA
CGCCTCGTCC GCATCGGCGA CAAATACGCG
CAGCAGGCTC GGCATGGCGG GCCAGTCCGT
GTGTCGGTCG AAGGCCAACA CGACGGTGTC
GCCGACTCGG ATCTCAGCGT GACCGATCAA
GCCCTCCTCG GTCGACACCC GCCCGAGTTC
CTCGCCCCCG AATGCCTGGG TGACGAAGTC
GAGGAAAGCC CCCGTGTCGT CGGTGACGAC
CCAGGGCGCA ACGGTGGTGT AGCCCTCCGG
CTCGGGGCTG GTCTGCTGGG GCATCGCGGT
CCTTTCGCTG ATGTTGCTTG CCAGCAGCGA
CGGTAGGCGT GAGCTAGGGT CAGCTTCTGT
CCTAGACGGC AGTGAGCTTC GAGCGCAACC
GGTACTACCT CACGACCGCT CGCGCTGATC
CAGACCCCGC CTCTGACCCC TGACCCCGTC
AGCCTGAGGT TGAAAAACGC T-3'
______________________________________
wherein A is deoxyadenyl C is deoxycytidyl G is deoxyguanyl, and T is thymidyl.
2. A recombinant DNA cloning vector which comprises the IS493 sequence of claim 1.
3. The recombinant DNA cloning vector of claim 2 which is a phage.
4. The recombinant DNA cloning vector of claim 2 which is a plasmid.
5. The recombinant DNA cloning vector of claim 4 that is plasmid pCZA141.
6. The recombinant DNA cloning vector of claim 4 that is plasmid pCZA150.
7. The recombinant DNA cloning vector of claim 4 that is plasmid pCZA151.
8. The recombinant DNA cloning vector of claim 4 that is plasmid pCZA152.
9. The recombinant DNA cloning vector of claim 4 that is plasmid pCZA153.
10. The recombinant DNA cloning vector of claim 4 that is plasmid pCZA154.
11. The recombinant DNA cloning vector of claim 4 that is plasmid pCZA155.Cited by (0)
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