[Leu13]motilin, DNAs coding for same and methods for producing same

Abstract

A novel motilin analog peptide containing leucine instead of the usual 13th amino acid methionine, as in the naturally occurring motilin, is produced by gene recombination techniques in which several or identical genes, each coding for the novel peptide, are joined in series into a vector. The resultant recombinant DNA is introduced into Escherichia coli and the resultant transformant is cultivated. The resulting polymeric peptide can be cleaved to give the desired peptide.

What is claimed is: 
     
       1. A peptide having the amino acid sequence: ##STR9## 
     
     
       2. A peptide having the amino acid sequence:   ______________________________________                                    
1    2      3      4    5    6    7    8    9    10                       
Phe  Val    Pro    Ile  Phe  Thr  Tyr  Gly  Glu  Leu                      
______________________________________                                    
11   12     13     14   15   16   17   18   19   20                       
Gln  Arg    Leu    Gln  Glu  Lys  Glu  Arg  Asn  Lys                      
______________________________________                                    
21   22                                                                   
Gly  Gln    Arg    Ile  Phe  Hse                                          
______________________________________                                    
     
     
     
       3. A polymeric peptide having the amino acid sequence:   ______________________________________                                    
[Phe Val Pro Ile Phe Thr Tyr Gly Glu Leu Gln Arg Leu Gln                  
Glu Lys Glu Arg Asn Lys Gly Gln Arg Ile Phe Met].sub.n                    
______________________________________                                    
     wherein n has a value of 2 to 32.   
     
     
       4. A pharmaceutical composition containing a therapeutically effective amount of the peptide of claim 1 together with a pharmaceutically acceptable carrier or diluent.