Process for purifying fructose 1,6-diphosphate

Abstract

A process for the purification of fructose 1,6-diphosphate which comprises subjecting a fructose 1,6-diphosphate-containing solution to anion exchange column chromatography to separate fructose 1,6-diphosphate therefrom, and subsequently subjecting the resulting FDP fraction to a desalting treatment, so as to provide a highly purified FDP preparation useful as a pharmaceutical drug and the like in a high yield.

What is claimed is: 
     
       1. A process for purifying fructose 1,6-diphosphate comprising the steps of: (A) adding a fructose 1,6-diphosphate-containing solution to an anion exchange column so as to absorb fructose 1,6-diphosphate onto said column;   (B) eluting said absorbed fructose 1,6-diphosphate from said anion exchange column to obtain a fructose 1,6-diphosphate fraction; and then   (C) subjecting the fructose 1,6-diphosphate fraction to a desalting treatment using a reverse osmosis membrane; so as to provide purified fructose 1,6-diphosphate.     
     
     
       2. The process for purifying fructose 1,6-diphosphate according to claim 1, wherein said fructose 1,6-diphosphate-containing solution to be added to the anion exchange column has a concentration of 1M or less. 
     
     
       3. The process for purifying fructose 1,6-diphosphate according to claim 2, wherein said fructose 1,6-diphosphate-containing solution to be added to the anion exchange column has a concentration of 100 mM or less. 
     
     
       4. The process for purifying fructose 1,6-diphosphate according to claim 3, wherein said fructose 1,6-diphosphate-containing solution to be added to the anion exchange column has a concentration of 10 mM or less. 
     
     
       5. The process for purifying fructose 1,6-diphosphate according to claim 1, wherein passage of the step (A) solution through said anion exchange column is effected at a liquid flow rate of 20 m/hr or less. 
     
     
       6. The process for purifying fructose 1,6-diphosphate according to claim 5, wherein passage of the step (A) solution through said anion exchange column is effected at a liquid flow rate of 6 m/hr or less. 
     
     
       7. The process for purifying fructose 1,6-diphosphate according to claim 6, wherein passage of the step (A) solution through said anion exchange column is effected at a liquid flow rate of around 2 m/hr. 
     
     
       8. The process for purifying fructose 1,6-diphosphate according to claim 1, wherein said absorbed fructose 1,6-diphosphate is eluted from the anion exchange column through the use of an inorganic salt aqueous solution. 
     
     
       9. The process for purifying fructose 1,6-diphosphate according to claim 8, wherein said inorganic salt aqueous solution is a sodium chloride aqueous solution. 
     
     
       10. The process for purifying fructose 1,6-diphosphate according to claim 1, wherein prior to step (A), said fructose 1,6-diphosphate-containing solution is initially purified to remove (i) phosphate impurities by means of a precipitant selected from the group consisting of barium chloride, zinc chloride, magnesium chloride, and calcium chloride, and (ii) proteinous impurities by means of ultrafiltration.