US5554369AExpiredUtilityPatentIndex 60
Method of controlling insects
Est. expirySep 23, 2011(expired)· nominal 20-yr term from priority
C12N 9/0006C12Y 101/03006C12N 15/8286A01N 63/10Y02A40/146
60
PatentIndex Score
3
Cited by
10
References
11
Claims
Abstract
3-Hydroxysteroid oxidase controls insects, particularly lepidopterans and boll weevil. Genes encoding for this enzyme may be cloned into vectors for transformation of plant-colonizing microorganisms or plants, thereby providing a method of controlling insect infestation.
Claims
exact text as granted — not AI-modifiedWhat is claimed is:
1. A method of controlling insect infestation of plants comprising providing a 3-hydroxysteroid oxidase for ingestion by the insect wherein the insect is a lepidopteran or boll weevil.
2. The method of claim 1 wherein the insect is in a larval stage.
3. The method of claim 1 wherein said 3-hydroxysteroid oxidase is the polypeptide of SEQ ID NO:8.
4. The method of claim 1 wherein said 3-hydroxysteroid oxidase is the polypeptide encoded by SEQ ID NO:13 or SEQ ID NO:14.
5. The method of claim 1 wherein said insect is boll weevil.
6. The method of claim 2 wherein said insect is boll weevil.
7. The method of claim I wherein said insect is a lepidopteran.
8. The method of claim 2 wherein said insect is a lepidopteran.
9. The method of claim 1 wherein said 3-hydroxysteroid oxidase is a Streptomyces cholesterol oxidase.
10. The method of claim 1 wherein said 3-hydroxysteroid oxidase is a Pseudomonas cholesterol oxidase.
11. The method of claim 1 wherein said 3-hydroxysteroid oxidase is a Brevibacterium cholesterol oxidase.Cited by (0)
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