Flavin reductase gene

Abstract

Nucleic acids coding for the flavin reductase of a luminescent bacterium, Xenorhabdus luminescens, vectors comprising such nucleic acids, host cells into which such vectors have been introduced, recombinant methods for producing substantially purified recombinant flavin reductase, and recombinantly produced flavin reductase and equivalent proteins produced in accordance with such methods are provided.

What is claim is: 
     
       1. A substantially purified nucleic acid comprising the nucleic acid sequence of SEQ ID NO:1. 
     
     
       2. A substantially purified nucleic acid comprising the nucleic acid sequence of SEQ ID NO:2. 
     
     
       3. A substantially purified nucleic acid comprising the nucleic acid sequence of SEQ ID NO:3. 
     
     
       4. A substantially purified nucleic acid comprising the nucleic acid sequence of SEQ ID NO:4. 
     
     
       5. A vector which comprises a nucleic acid of claim 1. 
     
     
       6. A vector which comprises a nucleic acid of claim 2. 
     
     
       7. A vector which comprises a nucleic acid of claim 3. 
     
     
       8. A vector which comprises a nucleic acid of claim 4. 
     
     
       9. A host cell containing the vector of claim 5. 
     
     
       10. A host cell containing the vector of claim 6. 
     
     
       11. A host cell containing the vector of claim 7. 
     
     
       12. A host cell containing the vector of claim 8. 
     
     
       13. A method of producing a protein which comprises the amino acid sequence of SEQ ID NO:5, wherein: (a) a vector comprising a nucleic acid of claim 1 is introduced in a host cell,   (b) the host cell is grown under conditions wherein said protein is produced, and   (c) said protein is isolated from said host cell.   
     
     
       14. A method of producing a protein which comprises the amino acid sequence of SEQ ID NO:5, wherein: (a) a vector comprising a nucleic acid of claim 2 is introduced in a host cell,   (b) the host cell is grown under conditions wherein said protein is produced, and   (c) said protein is isolated from said host cell.   
     
     
       15. A method of producing a protein which comprises the amino acid sequence of SEQ ID NO:5, wherein: (a) a vector comprising a nucleic acid of claim 3 is introduced in a host cell,   (b) the host cell is grown under conditions wherein said protein is produced, and   (c) said protein is isolated from said host cell.   
     
     
       16. A method of producing a protein which comprises the amino acid sequence of SEQ ID NO:5, wherein: (a) a vector comprising a nucleic acid of claim 4 is introduced in a host cell,   (b) the host cell is grown under conditions wherein said protein is produced, and   (c) said protein is isolated from said host cell.