D-amino acid oxidase from F. solani and DNA therefor

Abstract

D-amino acid oxidase (DAO) originated from Fusarium solani M-0718, a DNA compound encoding the oxidase, expression vectors containing said DNA compound are provided. Esherichia coli transformants capable of producing recombinant DAO in high level are also provided.

We claim: 
     
       1. An isolated DNA molecule which encodes a D-amino acid oxidase of F. solani, set forth in SEQ ID NO. 3. 
     
     
       2. The DNA of claim 1, wherein said DNA molecule comprises the nucleotide sequence set forth in SEQ ID NO: 2. 
     
     
       3. An expression vector which contains a DNA molecule of claim 1 or 2. 
     
     
       4. An microorganism transformed by a expression vector of claim 3. 
     
     
       5. The microorganism of claim 4 which is Escherichia coli.