B1k chain of laminin and methods of use

Abstract

Recombinant laminin B1k and fragments thereof.

What is claimed is: 
     
       1. A purified DNA which encodes a fragment of the B1k chain of laminin of SEQ ID NO:2 said fragment including all or part of domain VI and said fragment being at least 30 amino acid residues in length, provided that said fragment in not part of a full length B1k sequence and said fragment has the ability to bind to a K-laminin A chain. 
     
     
       2. A vector comprising the purified DNA sequence of claim 1, provided that said fragment in not part of a full length B1k sequence. 
     
     
       3. A cell containing the purified DNA of claim 2. 
     
     
       4. A method for manufacture of a fragment of the B1k chain of laminin of SEQ ID NO:2 said fragment including all or part of domain VI and said fragment being at least 30 amino acid residues in length, provided that said fragment in not part of a full length B1k sequence and said fragment has the ability to bind to a K-laminin A chain, comprising culturing the cell of claim 3 in a medium to express said B1k. 
     
     
       5. A cell containing the purified DNA of claim 1. 
     
     
       6. A method for manufacture of a a fragment of the B1k chain of laminin of SEQ ID NO:2 said fragment including all or part of domain VI and said fragment being at least 30 amino acid residues in length, provided that said fragment in not part of a full length B1k sequence and said fragment has the ability to bind to a K-laminin A chain, comprising culturing the cell of claim 5 in a medium to express said B1k fragment. 
     
     
       7. The purified DNA of claim 1, wherein said fragment is at least 60 amino acid residues in length. 
     
     
       8. The purified DNA of claim 1, wherein said fragment is at least 100 amino acid residues in length. 
     
     
       9. The purified DNA of claim 1, wherein said fragment is at least 200 amino acid residues in length.