Protein kinases

Abstract

Protein kinase mutant and wild-type genes encoding polypeptides of the class heretofore designated "casein kinase I" and useful in screening compositions which may affect DNA double-strand break repair activity are disclosed. Also disclosed are methods using the polynucleotides in cell-proliferative disorders.

I claim: 
     
       1. An isolated and purified polynucleotide sequence encoding a polypeptide selected from the group consisting of the polypeptide disclosed in SEQ ID NOs: 4, 6, 8, 10, 12, and 24. 
     
     
       2. The polynucleotide of claim 1 wherein the encoded polypeptide possesses casein kinase activity. 
     
     
       3. The polynucleotide of claim 1 wherein the encoded polypeptide possesses protein-serine/threonine kinase activity. 
     
     
       4. The polynucleotide of claim 1 wherein the encoded polypeptide possesses protein-tyrosine kinase activity. 
     
     
       5. The polynucleotide of claim 1 wherein the encoded polypeptide possess protein-serine/threonine and protein-tyrosine kinase activity. 
     
     
       6. The polynucleotide of claim 1, wherein the polypeptide is characterized as: a) promoting normal mitotic recombination; and   b) promoting the repair a DNA strand break which occurs at the cleavage site: ##STR3##   
     
     
       7. The polynucleotide of claim 1, selected from the group consisting of RNA, mRNA, genomic DNA and cDNA. 
     
     
       8. The polynucleotide of claim 1, selected from the group consisting of the DNA sequences of SEQ ID NOs. 3, 5, 7, 9, 11 and 23. 
     
     
       9. An autonomously replicating extrachromosomal DNA vector comprising a DNA according to claim 7. 
     
     
       10. A procaryotic or eucaryotic host cell stably transformed or transfected with a DNA according to claim 7. 
     
     
       11. A method for the production of a polypeptide possessing protein kinase and/or recombination/repair promoting activity comprising growing a host cell according to claim 10 in a suitable nutrient medium and isolating the desired polypeptide from said host cell or from the medium of its growth.