Human neutralizing monoclonal antibodies to human immunodeficiency virus

Abstract

The present invention describes human monoclonal antibodies which immunoreact with and neutralize human immunodeficiency virus (HIV). Also disclosed are immunotherapeutic and diagnostic methods of using the monoclonal antibodies, as well as cell line for producing the monoclonal antibodies.

What is claimed is: 
     
       1. A polynucleotide encoding a heavy chain immunoglobulin variable region amino acid residue sequence portion of a human monoclonal antibody capable of immunoreacting with human immunodeficiency virus (HIV) glycoprotein gp120 and neutralizing HIV, wherein the amino acid sequence is SEQ ID NO:66. 
     
     
       2. The polynucleotide of claim 1 wherein said polynucleotide is a deoxyribonucleotide. 
     
     
       3. A polynucleotide encoding a light chain immunoglobulin variable region amino acid residue sequence portion of a human monoclonal antibody capable of immunoreacting with human immunodeficiency virus (HIV) glycoprotein gp120 and neutralizing HIV, wherein the amino acid sequence is SEQ ID NO:97. 
     
     
       4. The polynucleotide of claim 3 wherein said polynucleotide is a deoxyribonucleotide. 
     
     
       5. A DNA expression vector comprising the polynucleotide of claim 1 or claim 3. 
     
     
       6. A DNA expression vector comprising the polynucleotides of claim 1 and claim 3. 
     
     
       7. The DNA expression vector of claim 6 wherein said monoclonal antibody has a heavy and light chain immunoglobulin variable region amino acid residue sequence as shown in the pair of SEQ ID NOs 66:97. 
     
     
       8. A host cell comprising the DNA expression vector of claim 5.