Immunoreactive peptide sequence from a 43 kD human cancer antigen

Abstract

PCT No. PCT/US94/09798 Sec. 371 Date Apr. 29, 1996 Sec. 102(e) Date Apr. 29, 1996 PCT Filed Aug. 30, 1994 PCT Pub. No. WO95/06725 PCT Pub. Date Mar. 9, 1995A recombinant cell line expressing a polypeptide with the amino acid sequence -Gln-Asp-Leu-Thr-Met-Lys-Tyr-Gln-Ile- has been isolated. The cell line was described as a recombinant E. coli strain. Isolated purified polypeptide include 10 or less amino acid sequences to a length of less than 100 amino acid sequences. The polypeptide is further defined as including the amino acid sequence of beta-galactosidase or glutathione-S-transferase.

We claim: 
     
       1. A recombinant cell line expressing a polypeptide wherein said polypeptide includes the amino acid sequence QDLTMKYQI (SEQ ID NO:9). 
     
     
       2. The cell line of claim 1 further defined as an E. coli strain. 
     
     
       3. The cell line of claim 1, wherein the expressed polypeptide includes the amino acid sequence QDLTMKYQIF (SEQ ID NO:1). 
     
     
       4. The cell line of claim 1, wherein the expressed polypeptide has a molecular mass of about 43 kDa, as measured by mobility through a denaturing polyacrylamide gel. 
     
     
       5. The cell line of claim 1, wherein the expressed polypeptide is further defined as having a length of less than 100 amino acids. 
     
     
       6. The cell line of claim 5, wherein the expressed polypeptide is further defined as having a length of less than 50 amino acids. 
     
     
       7. The cell line of claim 6, wherein the expressed polypeptide is further defined as having a length of less than 25 amino acids. 
     
     
       8. The cell line of claim 7, wherein the expressed polypeptide is further defined as having a length of 10 amino acids or less. 
     
     
       9. The cell line of claim 1, wherein the expressed polypeptide is further defined as including the amino acid sequence of β-galactosidase or glutathione-S-transferase.