Tetracycline resistance protein

Abstract

The invention provides tetracycline resistance protein polypeptides and DNA (RNA) encoding tetracycline resistance protein polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing tetracycline resistance protein polypeptides to screen for antibacterial compounds.

What is claimed is: 
     
       1. An isolated polynucleotide segment encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:2. 
     
     
       2. An isolated nucleic acid segment comprising a nucleotide sequence which is fully complementary to the polynucleotide segment of claim 1. 
     
     
       3. An isolated vector comprising the polynucleotide segment of claim 1. 
     
     
       4. An isolated vector comprising the nucleic acid of claim 2. 
     
     
       5. An isolated host cell comprising the vector of claim 3. 
     
     
       6. An isolated host cell comprising the vector of claim 4. 
     
     
       7. A process for producing a tetracycline resistance polypeptide encoded by said polynucleotide segment comprising culturing the host cell of claim 5 under conditions sufficient for the production of said polypeptide. 
     
     
       8. An isolated polynucleotide segment comprising a nucleotide sequence which is identical to the reference sequence of SEQ ID NO:1, except that the nucleotide sequence includes up to five nucleotide substitutions, insertions or deletions for every 100 nucleotides of the reference sequence of SEQ ID NO:1. 
     
     
       9. An isolated polynucleotide segment comprising a nucleotide sequence which is identical to the reference sequence of SEQ ID NO:1, except that the nucleotide sequence includes up to ten nucleotide substitutions, insertions or deletions for every 100 nucleotides of the reference sequence of SEQ ID NO:1. 
     
     
       10. An isolated polynucleotide segment comprising a nucleotide sequence encoding the same mature polypeptide expressed by the gene corresponding to the sequence of SEQ ID NO:1 contained in Staphylococcus aureus WCUH 29 contained in NCIMB Deposit Number 40771. 
     
     
       11. An isolated polynucleotide segment comprising a nucleotide sequence from position 1 to 1386 inclusive of the polynucleotide sequence set forth in SEQ ID NO:1. 
     
     
       12. An isolated nucleic acid segment comprising a nucleotide sequence which is fully complementary to the polynucleotide segment of claim 8,9,10 or 11. 
     
     
       13. An isolated vector comprising the polynucleotide segment of claim 8,9,10 or 11. 
     
     
       14. An isolated vector comprising the nucleic acid segment of claim 12. 
     
     
       15. An isolated host cell comprising the vector of claim 13. 
     
     
       16. An isolated host cell comprising the vector of claim 14. 
     
     
       17. A process for producing a tetracycline resistance polypeptide encoded by said polynucleotide segment comprising culturing the host cell of claim 15 under conditions sufficient for the production of said polypeptide.