US5891733AExpiredUtility

Reagent for analyzing solid components in urine and method for analyzing solid components by employing the same

90
Assignee: TOA MEDICAL ELECTRONICSPriority: Oct 20, 1994Filed: Oct 20, 1995Granted: Apr 6, 1999
Est. expiryOct 20, 2014(expired)· nominal 20-yr term from priority
Inventors:Junya Inoue
Y10T436/107497Y10T436/108331G01N 2015/0053Y10T436/101666G01N 2015/1477G01N 33/52Y10T436/10G01N 15/14Y10T436/11G01N 15/12Y10T436/106664Y10T436/117497G01N 33/569G01N 2015/1019
90
PatentIndex Score
84
Cited by
37
References
20
Claims

Abstract

A reagent for analyzing solid components in urine comprising: (i) a buffer agent for maintaining pH at 5.0 to 9.0, (ii) an osmotic pressure compensating agent for maintaining osmotic pressure at 100 mOsm/kg to 600 mOsm/kg, (iii) a first dye which is a condensed benzene derivative, (iv) a second fluorescent dye capable of staining a damaged cell, and (v) a chelating agent.

Claims

exact text as granted — not AI-modified
What is claimed is;: 
     
       1. A method capable of differentiating at least crystalline components, erythrocytes, yeast-like fungi and leukocytes in urine which comprises the steps of: mixing the urine with a reagent for analyzing solid components in urine comprising: (i) a buffer agent for maintaining pH at 5.0 to 9.0,   (ii) an osmotic pressure compensating agent for maintaining osmotic pressure at 100 mOsm/kg to 600 mOsm/kg,   (iii) a first dye which is a condensed benzene derivative selected from the group consisting of the following formula: 3,3'-(di-n-hexyl)-2,2'-oxacarbocyanine iodide (NK-2280, DiOC6(3)) ##STR14## 2-(p-dimethylaminostyryl)-3-methylbenzooxazolium iodide (NK-528) ##STR15## 2-(p-dimethylaminostyryl)-1,3,3-trimethyl-3H-indolium iodide (NK-97) ##STR16##  and is capable of differentiating crystalline components, erythrocytes and yeast-like fungi,     (iv) a second fluorescent dye capable of staining a damaged cell, and   (v) a chelating agent     to stain the desired solid components in the urine, applying an excitation light to solid components in the above stained urine, and   measuring the scattered light and the fluorescent light emitted from the solid components.   
     
     
       2. A method according to claim 1, wherein an electric resistance signal is measured after the solid components in the urine are stained. 
     
     
       3. A method according to claim 1, wherein the reagent for analyzing is mixed with the urine after the reagent is previously warmed. 
     
     
       4. A method according to claim 1, wherein said buffer agent maintains pH at 6.5 to 7.5. 
     
     
       5. A method according to claim 4, wherein said buffer agent maintains pH at 6.8 to 7.2. 
     
     
       6. A method according to claim 1, wherein said osmotic pressure compensating agent maintains osmotic pressure at 150 mOsm/kg to 500 mOsm/kg. 
     
     
       7. A method according to claim 1, wherein the electrical conductivity of the reagent is adjusted to 1 to 10 mS/cm. 
     
     
       8. A method according to claim 7, wherein the electrical conductivity of the reagent is adjusted to 4 to 7 mS/cm. 
     
     
       9. A method according to claim 1, wherein the reagent for analyzing is previously warmed to 30° C. to 40° C. 
     
     
       10. A method according to claim 9, wherein the reagent for analyzing is previously warmed to 33° C. to 37° C. 
     
     
       11. A method capable of differentiating at least crystalline components, erythrocytes, yeast-like fungi and leukocytes in urine which comprises the steps of: mixing the urine with a reagent for analyzing solid components in urine comprising: (I) a buffer agent for maintaining pH at 5.0 to 9.0,   (II) an osmotic pressure compensating agent for maintaining osmotic pressure at 100 mOsm/kg to 600 mOsm/kg,   (III) a dye capable of being excited by a red wave-length light, which is selected from the group consisting of the following: ##STR17##  Rhodnile Blue, Capri Blue BB, Basic Blue 124 and Basic Blue 1, and is capable of differentiating crystalline components, erythrocytes and yeast-like fungi, and   (IV) a chelating agent     to stain the desired solid components in the urine,   applying an excitation light to solid components in the above stained urine, and   measuring the scattered light and the fluorescent light emitted from the solid components.   
     
     
       12. A method according to claim 11, wherein an electric conductivity signal is measured after the solid components in the urine are stained. 
     
     
       13. A method according to claim 11, wherein the reagent for analyzing is mixed with the urine after the reagent is previously warmed. 
     
     
       14. A method according to claim 2, wherein said buffer agent maintains pH at 6.5 to 7.5. 
     
     
       15. A method according to claim 14, wherein said buffer agent maintains pH at 6.8 to 7.2. 
     
     
       16. A method according to claim 11, wherein said osmotic pressure compensating agent maintains osmotic pressure at 150 mOsm/kg to 500 mOsm/kg. 
     
     
       17. A method according to claim 11, wherein the electrical conductivity of the reagent is adjusted to 1 to 10 mS/cm. 
     
     
       18. A method according to claim 17, wherein the electrical conductivity of the reagent is adjusted to 4 to 7 mS/cm. 
     
     
       19. A method according to claim 11, wherein the reagent for analyzing is previously warmed to 30° C. to 40° C. 
     
     
       20. A method according to claim 19, wherein the reagent for analyzing is previously warmed to 33° C. to 37° C.

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