Compounds labeled with cyanate or thiocyanate metal complexes for detecting by infrared spectroscopy

Abstract

A labeled compound detectable by IR spectroscopy contains at least one histidine residue bound to a cyanate or thiocyanate metal complex that has an IR absorption band within the spectral region of 2399-1900 cm -1 . An assay reagent for simultaneously detecting or determining a plurality of different analytes in a sample is made up of a plurality of different labeled compounds, each being independently distinguishable from the others by absorbing energy in a different and distinguishable region in the range of 2300 to 1900 cm -1 .

What is claimed is: 
     
       1. An assay reagent for simultaneously detecting or determining a plurality of different analytes in a sample comprising a plurality of different labeled compounds, each being capable of entering into a bonding interaction for detecting and determining an analyte, wherein each labeled compound comprises a compound having at least one histidine group bound to an metal complex, the metal complex comprising a transition metal ion and at least one cyanate or thiocyanate ligand, and wherein the plurality of different labeled compounds are independently distinguishable from one another by each absorbing energy in a different and distinguishable region in the range of 2300 to 1900 cm -1 .