Activated recombinant adenovirus proteinases

Abstract

This application describes methods and expression constructs for producing activatable recombinant adenovirus proteinases. Purified activatable recombinant adenovirus proteinases and methods of purification are described. Activated adenovirus proteinases and methods for obtaining activated adenovirus proteinases are further included. Isolated peptide cofactors of adenovirus proteinase activity, methods of purifying and identifying said peptide cofactors are also described. Antibodies immunoreactive with adenovirus proteinases, immunospecific antibodies, and methods for preparing them are also described. Other related methods and materials are also described.

we claim: 
     
       1. A bacterial cell transformed with a plasmid selected from the group consisting of: a. pT7AD23K1;   b. pT7AD23K2;   c. pT7AD23K5;   d. pT7AD23k6 (ATCC Deposit No. 68322);   e. pT7AD23K8;   f. pT7AD23K10;   g. pT7AD23K11;   h. pT7AD23K13;   i. pT7AD23k15;   j. pT7AD23K16; and   k. pT7AD23K18.   
     
     
       2. An isolated nucleic acid having the nucleotide sequence SEQ ID NO: 39. 
     
     
       3. The plasmid pT7AD23K18.