DNA encoding gidA1 polypeptides

Abstract

The invention provides gidA1 polypeptides and DNA (RNA) encoding gidA1 polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing gidA1 polypeptides to screen for antibacterial compounds.

What is claimed is: 
     
       1. An isolated polynucleotide segment comprising: a first polynucleotide sequence, or the full complement of the entire length of the first polynucleotide sequence, wherein the first polynucleotide sequence is selected from the group consisting of: (a) a polynucleotide consisting of SEQ ID NO:1; and,   (b) a nucleic acid sequence identical to the polynucleotide of (a) except that, over the entire length corresponding to the polynucleotide of (a), up to thirty nucleotides are substituted, deleted or inserted for every 100 nucleotides of the polynucleotide of (a).   
     
     
       2. A vector comprising the isolated polynucleotide segment of claim 1. 
     
     
       3. An isolated polynucleotide segment comprising the vector of claim 2. 
     
     
       4. The isolated polynucleotide segment of claim 1, wherein the first polynucleotide sequence is selected from the group consisting of, the polynucleotide of (a); and, a nucleic acid sequence identical to the polynucleotide of (a) except that, over the entire length corresponding to the polynucleotide of (a), up to ten nucleotides are substituted, deleted or inserted for every 100 nucleotides of the polynucleotide of (a). 
     
     
       5. A vector comprising the isolated polynucleotide segment of claim 4. 
     
     
       6. An isolated host cell comprising the vector of claim 5. 
     
     
       7. The isolated polynucleotide segment of claim 1, wherein the first polynucleotide sequence is selected from the group consisting of: the polynucleotide of (a); and, a nucleic acid sequence identical to the polynucleotide of (a) except that, over the entire length corresponding to the polynucleotide of (a), up to five nucleotides are substituted, deleted or inserted for every 100 nucleotides of the polynucleotide of (a). 
     
     
       8. A vector comprising the isolated polynucleotide segment of claim 7. 
     
     
       9. An isolated host cell comprising the vector of claim 8. 
     
     
       10. The isolated polynucleotide segment of claim 1, wherein the first polynucleotide sequence is the polynucleotide of (a). 
     
     
       11. A vector comprising the isolated polynucleotide segment of claim 10. 
     
     
       12. An isolated host cell comprising the vector of claim 11. 
     
     
       13. An isolated polynucleotide segment, comprising a first polynucleotide sequence, or the full complement of the entire length of the first polynucleotide sequence, wherein the first polynucleotide sequence is selected from the group consisting of (a) a polynucleotide which encodes the same mature polypeptide, expressed by the gidA1 gene expressed by a polynucleotide comprising nucleotides 535 to 2364 of SEQ ID NO:1 contained in Chlaymydia trachomatis; and,   (b) a nucleic acid sequence identical to the polynucleotide of (a) except that, over the entire length corresponding to the polynucleotide of (a), up to twenty nucleotides are substituted, deleted or inserted for every 100 nucleotides of the polynucleotide of (a).   
     
     
       14. The isolated polynucleotide segment of claim 13, wherein the first polynucleotide sequence is selected from the group consisting of: the polynucleotide of (a); and, a nucleic acid sequence identical to the polynucleotide of (a) except that, over the entire length corresponding to the polynucleotide of (a), up to ten nucleotides are substituted, deleted or inserted for every 100 nucleotides of the polynucleotide of (a). 
     
     
       15. The isolated polynucleotide segment of claim 13, wherein the first polynucleotide sequence is selected from the group consisting of: the polynucleotide of (a); and, a nucleic acid sequence identical to the polynucleotide of (a) except that, over the entire length corresponding to the polynucleotide of (a), up to five nucleotides are substituted, deleted or inserted for every 100 nucleotides of the polynucleotide of (a). 
     
     
       16. The isolated polynucleotide segment of claim 13, wherein the first polynucleotide sequence is selected from the group consisting of: the polynucleotide of (a); and, a nucleic acid sequence identical to the polynucleotide of (a) except that, over the entire length corresponding to the polynucleotide of (a), up to three nucleotides are substituted, deleted or inserted for every 100 nucleotides of the polynucleotide of (a). 
     
     
       17. The isolated polynucleotide segment of claim 13, wherein the first polynucleotide sequence is the polynucleotide of (a). 
     
     
       18. A polynucleotide which encodes a fusion polypeptide and which includes the isolated polynucleotide segment according to claim 17. 
     
     
       19. An isolated polynucleotide segment, comprising a first polynucleotide sequence or the full complement of the entire length of the first polynucleotide sequence, wherein the first polynucleotide sequence hybridizes to the full complement of a reference polynucleotide comprising nucleotides 535 to 2354 of SEQ ID NO:1, wherein the hybridization conditions include incubation at 42° C. in a solution comprising: 50% formanide, 5×SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH7.6), 5×Denhardt's solution, 10% dextran sulfate, and 20 micrograms/ml denatured, sheared salmon sperm DNA, followed by washing in 0.1×SSC at about 65° C. 
     
     
       20. The isolated polynucleotide segment of claim 19, wherein the first polynucleotide sequence is identical to the reference polynucleotide except that, over the entire length corresponding to the reference polynucleotide, up to five nucleotides are substituted, deleted or inserted for every 100 nucleotides of the reference polynucleotide. 
     
     
       21. The isolated polynucleotide segment of claim 19, wherein the first polynucleotide sequence is identical to the reference polynucleotide except that, over the entire length corresponding to the reference polynucleotide, up to three nucleotides are substituted, deleted or inserted for every 100 nucleotides of the reference polynucleotide. 
     
     
       22. An isolated polynucleotide segment, comprising a first polynucleotide sequence or the full complement of the entire length of the first polynucleotide sequence, wherein the first polynucleotide sequence is selected from the group consisting of: (a) a polynucleotide which encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2; and,   (b) a nucleic acid sequence identical to the polynucleotide of (a) except that, over the entire length corresponding to the polynucleotide of (a), up to five nucleotides are substituted, deleted or inserted for every 100 nucleotides of the polynucleotide of (a).   
     
     
       23. A vector comprising the isolated polynucleotide segment of claim 22. 
     
     
       24. An isolated host cell comprising the vector of claim 23. 
     
     
       25. The isolated polynucleotide segment of claim 22, wherein the first polynucleotide is the polynucleotide of (a). 
     
     
       26. A vector comprising the isolated polynucleotide segment of claim 25. 
     
     
       27. An isolated host cell comprising the vector of claim 26. 
     
     
       28. A process for producing the polypeptide, comprising the step of culturing the host cell of claim 27 under conditions sufficient for the production of the polypeptide.