Nucleic acids encoding HST 2 muteins

Abstract

The present invention provides a polypeptide represented by the following amino acid sequence: - (Met)n=X=Leu=Ala=Gly=Glu=Ile=Ala=Gly=Val=Asn - - Trp=Glu=Ser - - Gly=Tyr=Leu=Val=Gly=Ile=Lys=Arg=Gln=Arg=Arg - - Leu=Tyr=Cys=Asn - - Val=Gly=Ile=Gly=Phe=His=Leu=Gln=Val=Leu=Pro - - Asp=Gly=Arg=Ile - - Ser=Gly=Thr=His=Glu=Glu=Asn=Pro=Tyr=Ser=Leu - - Leu=Glu=Ile=Ser - - Thr=Val=Glu=Arg=Gly=Val=Val=Ser=Leu=Phe=Gly - - Val=Arg=Ser=Ala - - Leu=Phe=Val=Ala=Met=Asn=Ser=Lys=Gly=Arg=Leu - - Tyr=Ala=Thr=Pro - - Ser=Phe=Gin=Glu=Glu=Cys=Lys=Phe=Arg=Glu=Thr - - Leu=Leu=Pro=Asn - - Asn=Tyr=Asn=Ala=Tyr=Glu=Ser=Asp=Leu=Tyr=Gln - - Gly=Thr=Tyr=Ile - - Ala=Leu=Ser=Lys=Tyr=Gly=Arg=Val=Lys=Arg=Gly - - Ser=Lys=Val=Ser - - Pro=Ile=Met=Thr=Val=Thr=His=Phe=Leu=Pro=Arg=Ile - wherein n is 0 or 1 and X represents Pro Ala Gly Thr Arg Ala Asn Asn Thr Leu Leu Asp Ser Arg Gly Trp Gly Thr Leu Leu Ser Arg Ser Arg Ala Gly or a fragment thereof (n=0: SEQ ID NO: 1, n=1: SEQ ID NO: 2), a recombinant DNA coding for the polypeptide, a vector containing the recombinant DNA, the preparation of a transformant carrying the vector, and the production of the polypeptide with the transformant.

What is claimed is: 
     
       1. A DNA sequence encoding a polypeptide represented by the following amino acid sequence: (Met)n X-(amino acid residue 27-170 of SEQ ID NO: 1)   wherein n is 0 or 1 and X represents X', wherein X' has the sequence of amino acid residues 1-26 of SEQ ID NO: 1, or is a fragment of X' that has at least one amino acid from the C-terminus of X'; and which has heparin-binding secretory transforming factor 2 (hst-2) activity as determined by measuring the stimulation of DNA synthesis by BALB/c3T3 cells or growth promotion of vascular endothelial cells.   
     
     
       2. The DNA sequence encoding the polypeptide as claimed in claim 1, wherein said polypeptide has amino acid sequence of SEQ ID NO: 1. 
     
     
       3. The DNA sequence encoding the polypeptide as claimed in claim 1, wherein said polypeptide has amino acid sequence of SEQ ID NO: 3. 
     
     
       4. The DNA sequence encoding the polypeptide as claimed in claim 1, wherein X represents Pro Ala Gly Thr Arg Ala Asn Asn Thr Leu Leu Asp Ser Arg Gly Trp Gly Thr Leu Leu Ser Arg Ser Arg Ala Gly, wherein when n is 0, the polypeptide is SEQ ID NO: 1 and when n is 1, the polypeptide is SEQ ID NO: 2. 
     
     
       5. The DNA encoding the polypeptide as claimed in claim 1, wherein X represents Gly, wherein when n is 0, the polypeptide is SEQ ID NO: 3, and when n is 1, the polypeptide is SEQ ID NO: 4. 
     
     
       6. A DNA sequence encoding a polypeptide represented by the following amino acid sequence: (Met)n X-(amino acid residues 27-170 of SEQ ID NO: 1)   wherein n is 0 or 1 and X represents X', wherein X' has the sequence of amino acid residues 1-26 of SEQ ID NO: 1, or a fragment of X', wherein 1 to 25 successive amino acid residues are deleted from the N-terminus of X',   and wherein said polypeptide has heparin-binding secretory transforming factor 2 (hst-2) activity as determined by measuring the stimulation of DNA synthesis by BALB/c3T3 cells or growth promotion of vascular endothelial cells.   
     
     
       7. A vector containing the recombinant DNA as claimed in any of claims 1-6. 
     
     
       8. A transformant carrying the vector as claimed in claim 7.