Polynucleotides encoding human G-protein chemokine receptor HDGNR10

Abstract

Human G-protein chemokine receptor polypeptides and DNA (RNA) encoding such polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptides for identifying antagonists and agonists to such polypeptides and methods of using the agonists and antagonists therapeutically to treat conditions related to the underexpression and overexpression of the G-protein chemokine receptor polypeptides, respectively. Also disclosed are diagnostic methods for detecting a mutation in the G-protein chemokine receptor nucleic acid sequences and detecting a level of the soluble form of the receptors in a sample derived from a host.

What is claimed is: 
     
       1. An isolated nucleic acid molecule comprising a polynucleotide selected from the group consisting of: (a) a polynucleotide encoding amino acids 1 to 352 of SEQ ID NO:2;   (b) a polynucleotide encoding amino acids 2 to 352 of SEQ ID NO:2;   (c) a polynucleotide encoding a polypeptide having the amino acid sequence encoded by the cDNA contained in ATCC Deposit No. 97183;   (d) the complement of (a);   (e) the complement of (b); and   (f) the complement of (c).   
     
     
       2. The isolated nucleic acid molecule of claim 1, wherein said polynucleotide is (a). 
     
     
       3. The isolated nucleic acid molecule of claim 1, wherein said polynucleotide is (b). 
     
     
       4. The isolated nucleic acid molecule of claim 1, wherein said polynucleotide is (c). 
     
     
       5. The isolated nucleic acid molecule of claim 1, wherein said polynucleotide is (d). 
     
     
       6. The isolated nucleic acid molecule of claim 1, wherein said polynucleotide is (e). 
     
     
       7. The isolated nucleic acid molecule of claim 1, wherein said polynucleotide is (f). 
     
     
       8. An isolated nucleic acid molecule comprising a polynucleotide encoding at least 50 contiguous amino acids of SEQ ID NO:2. 
     
     
       9. The isolated nucleic acid molecule of claim 2, which comprises nucleotides 259 to 1314 of SEQ ID NO:1. 
     
     
       10. The isolated nucleic acid molecule of claim 3, which comprises nucleotides 262 to 1314 of SEQ ID NO:1. 
     
     
       11. The isolated nucleic acid molecule of claim 1, which is DNA. 
     
     
       12. The isolated nucleic acid molecule of claim 1, which is RNA. 
     
     
       13. The isolated nucleic acid molecule of claim 1, wherein said polynucleotide is fused to a heterologous polynucleotide. 
     
     
       14. The isolated nucleic acid molecule of claim 1, wherein said heterologous polynucleotide encodes a heterologous polypeptide. 
     
     
       15. A recombinant vector comprising the nucleic acid molecule of claim 1. 
     
     
       16. A genetically engineered host cell that comprises the nucleic acid molecule of claim 1. 
     
     
       17. A genetically engineered host cell that comprises the nucleic acid molecule of claim 1 operatively associated with a regulatory sequence that controls gene expression. 
     
     
       18. A recombinant method for producing a polypeptide, comprising culturing the recombinant host cell of claim 1 under conditions such that said polypeptide is expressed and recovering said polypeptide. 
     
     
       19. An isolated nucleic acid molecule which maps to the same chromosome location as SEQ ID NO:1.