Manufacture of autogenous replacement body parts

Abstract

Disclosed are matrix materials, methods, and devices for manufacture in vivo of autogenous replacement body parts comprising plural distinct tissues. In one embodiment, the replacement body part is a skeletal joint and the new plural distinct tissues include bone and articular cartilage.

What is claimed is: 
     
       1. A device for forming a stable, functional, vitalized articulating skeletal joint at a joint defect site in a mammal, the device comprising: a biocompatible, bioresorbable, devitalized matrix, excised from a mammalian donor articulating skeletal joint and comprising,   an articulating surface, and,   plural distinct tissues including at least one non-mineralized tissue, said tissues capable of essentially maintaining their shape and relationships when used as a replacement joint, and having dimensions and structural relationships to each other which correspond anatomically to the articulating skeletal joint to be replaced, and, disposed on or within said matrix,   substantially pure exogenous osteogenic protein in an amount sufficient to induce formation of a new vitalized articulating surface and new vitalized plural distinct tissues thereby to permit regeneration of said stable, functional, vitalized articulating skeletal joint at said defect site.   
     
     
       2. A device for forming plural distinct non-mineralized tissues in a stable, functional, vitalized articulating skeletal joint in vivo, the device comprising: a biocompatible, bioresorbable, devitalized matrix excised from a mammalian donor articulating skeletal joint and comprising,   an articulating surface, and,   plural distinct non-mineralized tissues capable of essentially maintaining their shape and relationships when used as a replacement joint, said tissues having dimensions and structural relationships to each other which correspond anatomically to the articulating skeletal joint to be replaced, and, disposed on or within said matrix,   substantially pure exogenous osteogenic protein in an amount sufficient to induce formation of a new vitalized articulating surface and new vitalized plural distinct non-mineralized tissues thereby to permit regeneration of said stable, functional, vitalized articulating skeletal joint at said defect site.   
     
     
       3. The device of claim 1 wherein said plural distinct tissues comprise articular cartilage and bone. 
     
     
       4. The device of claim 1 or 2 wherein said non-mineralized skeletal joint tissue is selected from the group consisting of articular cartilage, ligament, tendon, intervertebral discs, joint capsule and synovial membrane tissue. 
     
     
       5. The device of claim 1 or 2 wherein said skeletal joint comprises a synovial joint. 
     
     
       6. The device of claim 1 or 2 wherein said non-mineralized tissue comprises an avascular tissue. 
     
     
       7. The device of claim 1 or 2 wherein said matrix comprises devitalized allogenic or xenogenic tissue. 
     
     
       8. The device of claim 1 or 2 wherein said device further comprises a matrix selected from the group consisting of: collagen; polymers comprising monomers of lactic acid, glycolic acid, butyric acid or derivatives thereof; and ceramics, hydroxyapatite, tricalcium phosphate, or mixtures thereof. 
     
     
       9. The device of claim 1 or 2 further comprising a material suitable for binding particulate matter to form a moldable solid. 
     
     
       10. The device of claim 1 or 2 wherein said exogenous osteogenic protein comprises homodimers or heterodimers of OP-1, OP-2, BMP2, BMP3, BMP4, BMP5, BMP6, OPX, or naturally sourced and recombinant derivatives of the foregoing. 
     
     
       11. A method for restoring a stable, functional, vitalized articulating skeletal joint at a joint defect site in mammal, the method comprising the step of: providing to the joint defect site, a device comprising a biocompatible, bioresorbable devitalized matrix, excised from a mammalian donor articulating skeletal joint and comprising,   an articulating surface, and,   plural distinct tissues including at least one non-mineralized tissue, said tissues capable of essentially maintaining their shape and relationships when used as a replacement joint, and having dimensions and structural relationships to each other which correspond anatomically to the articulating skeletal joint to be replaced, and disposed on or within said matrix,   substantially pure exogenous osteogenic protein in an amount sufficient to induce formation of a new vitalized articulating surface and new vitalized plural distinct tissues thereby to permit regeneration of said stable, functional, vitalized articulating skeletal joint at said defect site.   
     
     
       12. A method for restoring in a mammal, plural distinct non-mineralized tissues in a skeletal joint, the method comprising the step of: providing to the joint defect site, a device comprising biocompatible, bioresorbable, devitalized matrix, excised from a mammalian donor articulating skeletal joint and comprising,   an articulating surface, and,   plural distinct non-mineralized tissues capable of essentially maintaining their shape and relationships when used as a replacement joint, said tissues having dimensions and structural relationships to each other which correspond anatomically to the articulating skeletal joint to be replaced, and disposed on or within said matrix,   substantially pure exogenous osteogenic protein in an amount sufficient to induce formation of a new articulating surface and new plural distinct non-mineralized tissues thereby to permit regeneration of said stable, functional, vitalized articulating skeletal joint at said defect site.   
     
     
       13. The method of claim 11 wherein said plural distinct tissues comprise articular cartilage and bone. 
     
     
       14. The method of claim 11 or 12 wherein said matrix comprises devitalized allogenic or xenogenic tissue. 
     
     
       15. The method of claim 11 or 12 wherein said skeletal joint is a synovial joint. 
     
     
       16. The method of claim 11 or 12 wherein said non-mineralized tissue to be restored comprises avascular tissue. 
     
     
       17. The method of claim 11 or 12 wherein said non-mineralized tissue to be restored is selected from the group consisting of articular cartilage, tendon, ligament, intervertebral discs, joint capsule and synovial membrane tissue. 
     
     
       18. The method of claim 11 or 12 wherein said device further comprises a matrix selected from the group consisting of: collagen; polymers comprising lactic acid, butyric acid, glycolic acid or combinations thereof; and ceramics, tricalcium phosphate, hydroxyapatite or mixtures thereof. 
     
     
       19. The method of claim 11 or 12 wherein said exogenous osteogenic protein comprises homodimers or heterodimers of OP-1, OP-2, BMP2, BMP3, BMP4, BMP5, BMP6, OPX, or naturally sourced and recombinant derivatives of the foregoing.