Human pluripotent hematopoietic colony stimulating factor and cell line producing same

Abstract

Highly purified Pluripotent hematopoietic colony-stimulating factor (pluripotent CSF), a glycoprotein (MW 19,600) constitutively produced by human tumor cells has been highly purified from low serum-containing conditioned medium to apparant homogeneity. Pluripotent CSF supports the growth of human mixed colonies (CFU-GEMM), granulocyte-macrophage colonies (CFU-GM), early erythroid colonies (BFU-E) and induces differentiation of human leukemic cells. The specific activity of the purified pluripotent CSF in the CFU-GM assay is 1.5x108 U/mg protein.

What is claimed: 
     
       1. Purified subclonal cell line 1A6 isolated from a parent human tumor bladder cell line 5637. 
     
     
       2. The subclonal cell line of claim 1, wherein the purified subclonal cell line 1A6 produces between 2-10 fold higher amounts of p-CSF than the parent cell line, human tumor bladder cell line 5637.