Complementary DNA for rice chitinase having lytic activity against moulds and bacteria, and vector containing said complementary DNA and transformant

Abstract

The present invention provides a complementary DNA for a rice chitinase having a lytic activity against moulds and a bacteria, a complementary DNA for a rice chitinase encoding a protein comprising an amino acid sequence described in SEQ ID NO: 1 in SEQUENCE LISTING, a plasmid vector containing said complementary DNA and a transformant having said plasmid vector. The present invention enables realization of a possibility of developing a recombinant crop plant having an acquired resistance against a wide range of pathogenic microorganisms by identifying a complementary DNA encoding a novel chitinase capable of lysing cell walls of both of moulds and bacteria and introducing the sequence thereof into a plant.

What is claimed is: 
     
       1. A complementary DNA for a rice chitinase encoding a protein comprising an amino acid sequence described in SEQ ID NO: 6 in SEQUENCE LISTING. 
     
     
       2. A plasmid vector containing the complementary DNA for a rice chitinase according to claim 1. 
     
     
       3. A transformant having the plasmid vector according to claim 2. 
     
     
       4. The transformant according to claim 3 wherein said transformant is E. coli pS4960 (FERM BP-6286).