P
US6180387B1ExpiredUtilityPatentIndex 74

Arginine deiminase

Assignee: SMITHKLINE BEECHAM CORPPriority: Aug 11, 1997Filed: Nov 5, 1997Granted: Jan 30, 2001
Est. expiryAug 11, 2017(expired)· nominal 20-yr term from priority
Inventors:BISWAS SANJOYBURNHAM MARTIN KARL RUSSELLONETTO MICHAEL ARTHURWARREN PATRICK VERNONWARREN RICHARD LLOYD
A61P 9/00A61P 27/00A61P 31/00A61P 27/02A61P 25/00A61P 31/04A61P 19/00A61P 11/00A61P 17/00A61P 19/02A61K 38/00A61P 13/02A61P 13/00A61P 1/00A61P 13/12C12N 9/1096C12N 9/78A61K 39/00
74
PatentIndex Score
10
Cited by
6
References
26
Claims

Abstract

The invention provides arginine deiminase polypeptides and DNA (RNA) encoding arginine deiminase polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing arginine deiminase polypeptides to screen for antibacterial compounds.

Claims

exact text as granted — not AI-modified
What is claimed is:  
     
       1. An isolated polynucleotide fragment comprising a first polynucleotide sequence or the full complement of the entire length of the first polynucleotide sequence, wherein the first polynucleotide sequence encodes an arginine deiminase polypeptide comprising SEQ ID NO:2. 
     
     
       2. The isolated polynucleotide fragment of claim  1 , wherein the isolated polynucleotide fragment comprises the first polyleotide sequence. 
     
     
       3. A vector comprising the isolated polynucleotide fragment of claim  2 . 
     
     
       4. An isolated host cell comp sing the vector of claim  3 . 
     
     
       5. A process for producing an arginine deiminase polypeptide comprising culturing the host cell of claim  4  under conditions sufficient for the production of the polypeptide, wherein the polypeptide is encoded by the first polynucleotide sequence. 
     
     
       6. The isolated polynucleotide fragment of claim  1 , wherein the isolated polynucleotide fragment comprises the fuill complement of the entire length of the first polynucleotide sequence. 
     
     
       7. A vector comprising the isolated polynucleotide fragment of claim  6 . 
     
     
       8. An isolated host cell comprising the vector of claim  7 . 
     
     
       9. The isolated polynucleotide fragment of claim  7  encoding a fusion polypeptide, wherein the flsion polypeptide comprises SEQ ID NO:2. 
     
     
       10. An isolated polynucleotidc fragment encoding arginine deiminase comprising a first polynucleotde sequence or the full complement of the entire length of the first polynucleotide sequence, wherein the first polynucleotide sequence comprises SEQ ID NO:1. 
     
     
       11. The isolated polynucleotide fragment of claim  10 , wherein the isolated polynucleotide fragment comprises the first polynucleotide sequence. 
     
     
       12. A vector comprising the isolated polynucleotide fragment of claim  11 . 
     
     
       13. An isolated host cell comprising the vector of claim  12 . 
     
     
       14. A process for producing an arginine deiminase polypeptide comprising culturing the host cell of claim  13  under conditions sufficient for the production of the polypeptide, wherein the polypeptide is encoded by the first polynuclcotide sequence. 
     
     
       15. The isolated polynucleotide fragment of claim  10 , wherein the isolated polynucleotide fragment comprises the full complement of the entire length of the first polynucleotide sequence. 
     
     
       16. A vector comprising the isolated polynucleotide fragment of claim  15 . 
     
     
       17. An isolated host cell comprising the vector of claim  16 . 
     
     
       18. The isolated polynuclcotide fragment of claim  11  encoding a fusion polypeptide, wherein the fusion polypeptide comprises SEQ ID NO:2. 
     
     
       19. An isolated polynuclcotide fragment comprising a first polynucleotide sequence or the full complement of the entire length of the first polynucleotide sequence, wherein the first polynucleotide sequence encodes an arginine deiminase polypeptide consisting of SEQ ID NO:2. 
     
     
       20. The isolated polynucleotide fragment of claim  19 , wherein the isolated polynucleotide fragment comprises the first polynucleotide sequence. 
     
     
       21. A vector comprising the isolated polynucleotide fragment of claim  20 . 
     
     
       22. An isolated host cell comprising the vector of claim  21 . 
     
     
       23. A process for producing an arginine deiminase polypeptide comprising culturing the host cell of claim  22  under conditions sufficient for the production of the polypeptide, wherein the polypeptide is encoded by the fist polynucleotide sequence. 
     
     
       24. The isolated polynucleotide fragment of claim  19 , wherein the isolated polynucleotide fragment comprises the full complement of the entire length of the first polynucleotide sequence. 
     
     
       25. A vector comprising the isolated polynucleotide fragment of claim  24 . 
     
     
       26. An isolated host cell comprising the vector of claim  25 .

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