GidA1

Abstract

The invention provides gidA1 polypeptides and polynucleotides encoding gidA1 polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing gidA1 polypeptides to screen for antibacterial compounds.

What is claimed is:  
     
       1. An isolated polynucleotide segment comprising a first polynucleotide sequence or the full complement of the entire length of the first polynucleotide sequence, wherein the first polynucleotide sequence is identical to SEQ ID NO: 1, except that, over the entire length corresponding to SEQ ID NO: 1, n n  nucleotides are substituted, inserted or deleted, wherein n n  satisfies the following expression 
       
         
             n   n   ≦x   n −( x   n   ·y )  
         
       
       wherein x n  is the total number of nucleotides in SEQ ID NO:1, y is at least 0.97, and wherein any non-integer product of x n  and y is rounded down to the nearest integer before subtracting the product from x n ; and, wherein the first polynucleotide sequence detects  Streptococcus pneumoniae  by hybridization. 
     
     
       2. A vector comprising the isolated polynucleotide segment of claim  1 . 
     
     
       3. An isolated host cell comprising the vector of claim  2 . 
     
     
       4. The isolated polynucleotide segment of claim  1 , wherein y is at least 0.99. 
     
     
       5. An isolated polynucleotide segment comprising a first polynucleotide sequence or the full complement of the entire length of the first polynucleotide sequence, wherein the first polynucleotide sequence encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2. 
     
     
       6. A vector comprising the isolated polynucleotide segment of claim  5 . 
     
     
       7. An isolated host cell comprising the vector of claim  6 . 
     
     
       8. A process for producing a polypeptide comprising culturing the host cell of claim  7  under conditions sufficient for the production of the polypeptide, wherein the polypeptide is encoded by the first polynucleotide sequence. 
     
     
       9. The isolated polynucleotide of claim  5  encoding a fusion polypeptide, wherein the first polynucleotide encodes part of the fusion polypeptide. 
     
     
       10. An isolated polynucleotide segment comprising a first polynucleotide sequence or the full complement of the entire length of the first polynucleotide sequence, wherein the first polynucleotide sequence comprises the nucleotide sequence set forth in SEQ ID NO: 1. 
     
     
       11. A vector comprising the isolated polynucleotide segment of claim  10 . 
     
     
       12. An isolated host cell comprising the vector of claim  11 . 
     
     
       13. A process for producing a polypeptide comprising culturing the host cell of claim  12  under conditions sufficient for the production of the polypeptide, wherein the polypeptide is encoded by the first polynucleotide sequence. 
     
     
       14. The isolated polynucleotide of claim  10  encoding a fusion polypeptide, wherein the first polynucleotide encodes part of the fusion polypeptide. 
     
     
       15. An isolated polynucleotide segment comprising a first polynucleotide sequence or the full complement of the entire length of the first polynucleotide sequence, wherein the first polynucleotide sequence encodes a polypeptide consisting of the amino acid sequence set forth in SEQ ID NO:2. 
     
     
       16. A vector comprising the isolated polynucleotide segment of claim  15 . 
     
     
       17. An isolated host cell comprising the vector of claim  16 . 
     
     
       18. A process for producing a polypeptide comprising culturing the host cell of claim  17  under conditions sufficient for the production of the polypeptide, wherein the polypeptide is encoded by the first polynucleotide sequence.